Lee Young-Jin (ed.) Mass Spectrometry Imaging of Small Molecules: Methods and Protocols

Humana Press, 2022. — 270 р. — (Methods in Molecular Biology 2437). — ISBN 978-1-0716-2029-8.This volume presents updated methods and new developments in the field of mass spectrometry imaging. Chapters guide readers through four parts covering imaging, software, data analysis, new instrumentation, and new methodological approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mass Spectrometry Imaging of Small Molecules aims to be a useful practical guide to researchers to help further their study in this field.Innovations in Instrumental Methods for Mass Spectrometry Imaging
Gas-Phase Ion–Ion Reactions for Lipid Identification in Biological Tissue Sections
MALDI-2 and t-MALDI-2 Mass Spectrometry Imaging
Ambient Mass Spectrometry Imaging of Small Molecules from Cells and Tissues
Single-Cell Metabolomics with Rapid Determination of Chemical Formulas from Isotopic Fine Structures
TOF-SIMS Imaging of Biological Tissue Sections and Structural Determination Using Tandem MS
New Types of Laser Absorption Methods in Mass Spectrometry Imaging
Mass Spectrometry Imaging of Biological Tissues by Laser Desorption Ionization from Silicon Nanopost Arrays
Metal-Assisted Laser Desorption Ionization Imaging Mass Spectrometry
Mass Spectrometry Imaging of Metabolites by Nanostructure Initiator Mass Spectrometry with Fluorinated Gold Nanoparticles
Imaging of Plant Hormones with Nanoparticle-Assisted Laser Desorption/Ionization Mass Spectrometry
Quantitative Mass Spectrometry Imaging
Nanosecond Photochemical Reaction (nsPCR) for Enhanced Mass Spectrometric Identification, Quantification, and Visualization of Metabolites and Neuropeptides
Extraction and Upconcentration of Adsorbates from Precisely Defined Area for Quantitative MALDI Mass Spectrometry Imaging
Development and Application of DropletProbe Mass Spectrometry for Examining Biodistribution of Therapeutics
Quantitative Mass Spectrometry Imaging with Liquid Microjunction Surface Sampling
New Types of Applications
Enhancing Metabolite Coverage for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Through Multiple On-Tissue Chemical Derivatizations
Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Glycogen In Situ
MALDI Mass Spectrometry Imaging of Lipids on Free-Floating Brain Sections and Immunohistochemically Colocalized Markers of Neurodegeneration
Silver-Doped Nano-DESI MSI for Increased Specificity and Sensitivity of Alkenes
Innovations in Data Analysis
Extract Metabolomic Information from Mass Spectrometry Images Using Advanced Data Analysis