Del Valle Luis (ed.) Immunohistochemistry and Immunocytochemistry: Methods and Protocols

Humana Press, 2022. — 284 p. — (Methods in Molecular Biology 2422). — ISBN 978-1-0716-1947-6.This volume provides a comprehensive reference guide for researchers to study the applications of labeled antibodies. Chapters guide reader through the the and practice of immunohistochemistry, immunocytochemistry and immunofluorescence techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and useful tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Immunohistochemistry and Immunofluorescence: Methods and Protocols aims to be a useful practical guide to scientists to help further their study in this fieldIntroduction to Immunohistochemistry: From to Evolving Science to Timeless Art
Immunohistochemistry in Historical Perspective: Knowing the Past to Understand the Present
Sample Preparation and Fixation for Histology and Pathology
Tissue Processing
Antigen Retrieval and Signal Amplification
Primary Antibody Selection and Blocking Techniques for Immunohistochemistry
Probes for Fluorescent Visualization of Specific Cellular Organelles
Enzymatic Immunohistochemistry
Fluorescent Immunohistochemistry
Double Labeling Fluorescent Immunocytochemistry
Multiplexing and Spectral Microscopy
Fluorescent In Situ Hybridization (FISH)
Proximity Ligation Assay (PLA)
Immunohistochemistry, Quality Control, and Principles of Validation in the Central Nervous System
Culture and Phenotyping of Glial Cell Cultures, Gliospheres, and Neurospheres
ER+ Breast Cancer Mammosphere Formation and Analysis
Detection of Circulating and Tissue Myeloid-Derived Suppressor Cells (MDSC) by Flow Cytometry
Comet Assay for the Detection of Single and Double-Strand DNA Breaks
Fluorescent-Activated Cell Sorting (Flow Cytometry)