Rao Ayala L.N., Lavagi-Craddock Irene, Vidalakis Georgios (eds.) Viroids: Methods and Protocols

Humana Press, 2022. — 368 p. — (Methods in Molecular Biology 2316). — ISBN 978-1-0716-1463-1.This volume explores the latest methods used by researchers to study the detection, characterization, and various aspects of viroids. The chapters in this book are organized into seven parts and cover topics such as detection methods based on the biology of viroids; detection techniques based on electrophoresis and hybridization techniques; PCR-based techniques that provide high degrees of sensitivity; emerging area of nucleic acid sequence-based technology; and emerging techniques in viroid research such as RNA silencing, splicing, and viroid structure. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Viroids: Methods and Protocols is a valuable resource for researchers and graduate students who are working with viroid diseases.Biological Techniques
Study and Detection of Citrus Viroids in Woody Hosts
Detection and Characterization of Viroids via Biological Assays on Herbaceous Hosts
Analysis on RNA Motif-Based RNA Trafficking in Plants
Isolation and Transfection of Citrus Protoplasts with Citrus Exocortis Viroid
Electrophoresis and Hybridization Techniques
High-Throughput RNA Extraction from Citrus Tissues for the Detection of Viroids
Extraction and Purification of Viroids from Herbaceous Hosts
Detection of Viroids by sPAGE Gel Electrophoresis
Two-Dimensional Polyacrylamide Gel Electrophoresis Analysis of Viroid RNAs
A Simplified Dot-Blot Hybridization Protocol for Potato spindle tuber viroid Detection in Solanaceae
Gel Blot Hybridization for Viroids
Tissue Print Hybridization for Detection and Characterization of Viroids
Fluorescein-Based Electrophoretic Mobility Shift Assay
PCR Techniques
Detection of Viroids by RT-PCR
Detection of Viroids Using RT-qPCR
Multiplex RT-PCR
Real-Time Detection of Viroids Using Singleplex and Multiplex Quantitative Polymerase Chain Reaction
Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) at the Service of Viroid Detection
SYBR Green RT-qPCR for the Universal Detection of Citrus Viroids
Detection of Avocado Sunblotch and Other Viroids Using RNA Filter Paper Capture and RT-PCR
Cloning and Sequencing Techniques
Cloning and Sequencing of Viroids
QuantiGene Plex Assay: A Method for High-Throughput Multiplex Citrus Viroid Detection and Identification
Culture-Independent Discovery of Viroids by Deep Sequencing and Computational Algorithms
An In Silico Detection of a Citrus Viroid from Raw High-Throughput Sequencing Data
RNA Silencing Techniques
Detection of Viroid RNA and vd-siRNA in N. benthamiana Plants: Northern Blot Analyses for Viroid and vd-siRNAs
Splicing Techniques
Production of Recombinant RNA in Escherichia coli Using Eggplant Latent Viroid as a Scaffold
Structural Analysis
Predicting the Structure of a Viroid