Graca Luis (ed.) T-Follicular Helper Cells: Methods and Protocols

Humana Press, 2022. — 288 p. — (Methods in Molecular Biology 2380). — ISBN 978-1-0716-1735-9.The volume provides a comprehensive overview of methods that have been developed for the study of Tfh and Tfr biology in mice, and in different human diseases. The chapters detail methods to investigate the function of Tfh cells in vivo and in vitro, including the use of mathematical models to study GC dynamics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, T-Follicular Helper Cells: Methods and Protocols aims to be a useful practical guide to researchers to help further their study in this field.Identification of Tfh and Tfr Cells in Humans and Mice
Complete Visualization of T Follicular Helper Cells in Germinal Centers by Light Sheet Fluorescence Microscopy
In Vivo Imaging of Tfh Cells
Circulating T Follicular Helper Subsets in Human Blood
Identification of Human Blood and Tissue T Follicular Regulatory (Tfr) Cells by Flow Cytometry
Using Mass Cytometry to Address Tfh and Tfr Heterogeneity
Identification of T Peripheral Helper (Tph) Cells
Identification of Non-classical Follicular T Cells
Identification of Follicular T Cells in the Gut
Development and Function of Tfh and Tfr Cells
In Vivo Migration and Tfh Cell Interactions
A method to analyze models of the dynamics of Germinal Centers
Identification of Antigen Presenting Cell Subsets Supporting Human Tfh Differentiation
The Use of Peptide-MHCII Tetramers to Identify Antigen-Specific T Follicular Helper and T Follicular Regulatory Cells
Assessment of the TCR Repertoire of Human Circulating T Follicular Helper Cells
Assessing In Vivo T Cell-Dependent Antigen-Specific Antibody Responses
Tfh-Mediated and Tfr-Suppressed Antigen-Driven IgG and IgE Assays
Tfh and Tfr Cells in Infectious and Inflammatory Diseases
Assessment of the Impact of Cytokines on TFH, TREG, and TFR Cell Populations After Influenza Infection
Evaluation of Human Circulating T Follicular Helper Cells in Influenza- and SARS-CoV-2-Specific B Cell Immunity
Detection of T Follicular Helper Cells and T Follicular Regulatory Cells in Experimental Sjögren’s Syndrome
Identification of Human T Follicular Cells in Ectopic Lymphoid Structures
T Follicular Helper Cells in Asthma Through Murine Models of Allergic Airway Disease
Therapeutic Modulation of T Follicular Helper Cells by Low-Dose IL-2 Treatment
3D Tissue Explant and Single-Cell Suspension Organoid Culture Systems for Ex Vivo Drug Testing on Human Tonsil-Derived T Follicular Helper Cells