Shulaev Vladimir (ed.) Plant Metabolic Engineering: Methods and Protocols

Humana Press, 2022. — 506 p. — (Methods in Molecular Biology 2396). — ISBN 978-1-0716-1822-6.This volume looks at the latest techniques used by researchers to study various aspects of plant metabolic engineering. The chapters in this book cover topics such as bioinformatics tools used to discover new genes and pathways; heterologous expression of biosynthetic genes in plant and microbial systems; and omics technologies, such as transcriptomics, proteomics, metabolomics, and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Plant Metabolic Engineering: Methods and Protocols is a valuable resource for biologists, chemists, biotechnologists, students, and broad cohorts of researchers who works in the fields of plant metabolism and metabolic engineering.Methods for the Development of Recombinant Microorganisms for the Production of Natural Products
Sustainable Technological Methods for the Extraction of Phytochemicals from Citrus Byproducts
Reconstitution of Metabolic Pathway in Nicotiana benthamiana
A Protocol for Phylogenetic Reconstruction
RNA-Seq Data Analysis Pipeline for Plants:​ Transcriptome Assembly, Alignment, and Differential Expression Analysis
A Protocol for Horizontally Acquired Metabolic Gene Detection in Algae
Global Comparative Label-Free Yeast Proteome Analysis by LC-MS/​MS After High-pH Reversed-Phase Peptide Fractionation Using Solid-Phase Extraction Cartridges
Gas Chromatography Coupled to Atmospheric Pressure Chemical Ionization High-Resolution Mass Spectrometry for Metabolite Fingerprinting of Grape (Vitis vinifera L) Berry
GC-MS/​MS Profiling of Plant Metabolites
Analysis of Grape Volatiles Using Atmospheric Pressure Ionization Gas Chromatography Mass Spectrometry-Based Metabolomics
A High-Throughput HILIC-MS-Based Metabolomic Assay for the Analysis of Polar Metabolites
Macrolipidomic Profiling of Vegetable Oils:​ The Analysis of Sunflower Oils with Different Oleic Acid Content
Non-targeted Lipidomics Using a Robust and Reproducible Lipid Separation Using UPLC with Charged Surface Hybrid Technology and High-Resolution Mass Spectrometry
Comprehensive Analysis of Plant Lipids Using Sub-2-μm Particle CO2-Based Chromatography Coupled to Mass Spectrometry
Bioinformatics in Lipidomics:​ Automating Large-Scale LC-MS-Based Untargeted Lipidomics Profiling with SimLipid Software
A Protocol for Prion Discovery in Plants
Structural Determination of Uridine Diphosphate Glycosyltransfer​ases Using X-Ray Crystallography