Weissig Volkmar, Edeas Marvin (eds.) Mitochondrial Medicine. Volume 3: Manipulating Mitochondria and Disease- Specific Approaches

2nd edition. — Humana Press, 2021. — 462 p. — (Methods Molecular Biology 2277). — ISBN 978-1-0716-1269-9.This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume III guides readers through chapters on intercellular mitochondrial transfer, Cytoplasmic transfer methods, Ndufs4 knockout mouse, in vivo assessment of mitochondrial oxygen consumption, mitochondrial network analysis by imaging, mitochondrial respiration in platelets, Heterologous Inferential Analysis (HIA), bioenergetic profiling, integrative methods for studying cardiac energetics, and isolation and deep sequencing of mitochondrial DNA.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Mitochondrial Medicine, Second Edition, Volume 3: Manipulating Mitochondria and Disease- Specific Approaches aims to be a comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine.Allotopic Expression of ATP6 in Mouse as a Transgenic Model of Mitochondrial Disease
Mitochondrial Transplantation for Ischemia Reperfusion Injury
Quantitatively Controlled Intercellular Mitochondrial Transfer by Cell Fusion-Based Method Using a Microfluidic Device
Lipophilic Conjugates for Carrier-Free Delivery of RNA Importable into Human Mitochondria
Drug Discovery Assay to Identify Modulators of the Mitochondrial Ca2+ Uniporter
Cytoplasmic Transfer Methods for Studying the Segregation of Mitochondrial DNA in Mice
Electron Attachment to Isolated Molecules as a Probe to Understand Mitochondrial Reductive Processes
Assessment of Short- and Medium-Chain Fatty Acids on Mitochondrial Function in Severe Inflammation
Assessment of the Effects of Drugs on Mitochondrial Respiration
The NDUFS4 Knockout Mouse: A Dual Threat Model of Childhood Mitochondrial Disease and Normative Aging
Suborganellar Localization of Mitochondrial Proteins and Transcripts in Human Cells
In Vivo Assessment of Mitochondrial Oxygen Consumption
Update of Mitochondrial Network Analysis by Imaging: Proof of Technique in Schizophrenia
Heterologous Inferential Analysis (HIA) and Other Emerging Concepts: In Understanding Mitochondrial Variation In Pathogenesis: There is no More Low-Hanging Fruit
Accurate Measurement of Cellular and Cell-Free Circulating Mitochondrial DNA Content from Human Blood Samples Using Real-Time Quantitative PCR
Measurement of Mitochondrial Respiration in Platelets
Isolation and Electron Microscopic Analysis of Liver Cancer Cell Mitochondria
Assessment of Mitochondrial Reactive Oxygen Species and Redox Regulation in Stem Cells
Single-Cell Approaches for Studying the Role of Mitochondrial DNA in Neurodegenerative Disease
A New Method for Sequencing the Mitochondrial Genome by Using Long Read Technology
Mitochondrial DNA as a Sensitive Biomarker of UV-Induced Cellular Damage in Human Skin
Isolation of Mitochondria-Associated ER Membranes (MAMs), Synaptic MAMs, and Glycosphingolipid Enriched Microdomains (GEMs) from Brain
Tissues and Neuronal Cells
Mitochondrial Diagnostics: A Discovery-Based Biochemical Platform for Phenotyping Human Peripheral Blood Cell Mitochondria
Bioenergetic Profiling of Human Pluripotent Stem Cells
Integrative Methods for Studying Cardiac Energetics
Isolation of Mitochondria from Retinal Pigment Epithelial Cell Cultures and an Application of High-Resolution Respirometric Assay (XFe96 Seahorse Assay)
The Isolation and Deep Sequencing of Mitochondrial DNA
Ultrastructure of the Mitochondria-Associated Membranes in Human Tumor Specimens