Basu Chhandak (ed.) PCR Primer Design

3rd edition. — Humana Press, 2022. — 270 p. — (Methods in Molecular Biology 2392). — ISBN 978-1-0716-1798-4.This third edition provides new and updated chapters on design PCR primers for successful DNA amplification. Chapters are divided into seven parts, including primer design strategies for quantitative PCR, genotyping, multiplex PCR, in silico PCR primer design, and primer design to identify plant and animal viruses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, PCR Primer Design, Third Edition aims to be useful for various fields of molecular biology, including biotechnology, molecular genetics, and recombinant DNA technology.Primer Design for Genotyping
The Significance of PCR Primer Design in Genetic Diversity Studies: Exemplified by Recent Research into the Genetic Structure of Marine Species
Enhancing Cohort PASA Efficiency from Lessons Assimilated by Mutant Genotyping in C. elegans
Design of Oligonucleotides for Allele-Specific Amplification Based on PCR and Isothermal Techniques
Detection of Rubella Virus by Tri-Primer RT-PCR Assay and Genotyping by Fragment RT-PCR
Design of Mismatch Primers to Identify and Differentiate Closely Related (Sub)Species: Application to the Authentication of Meat Products
Primer Design for the Analysis of Closely Related Species: Application of Noncoding mtDNA and cpDNA Sequences
Designing PCR Primers for the Amplification-Refractory Mutation System
Primer Design for Genome-Wide Identification of Specific Regions
Validation of Circular RNAs by PCR
Primer Designing for Amplifying an AT-Rich Promoter from Arabidopsis thaliana
Primer Design for Multiplex PCR. Multiplex
PLASmid TAXonomic PCR (PlasTax-PCR), a Multiplex Relaxase MOB Typing to Assort Plasmids into Taxonomic Units
Multiplex PCR Design for Scalable Resequencing
Primer Design for qPCR
Identification of Gene Copy Number in the Transgenic Plants by Quantitative Polymerase Chain Reaction (qPCR)
Identification of Gene Copy Number in the Transgenic Plants by Quantitative Polymerase Chain Reaction (qPCR)
Primer Design for Identification of Plant and Animal Viruses
PCR Primer Design for the Rapidly Evolving SARS-CoV-2 Genome
Primer Design for Identification of Plant and Animal Viruses
Universal Primers for Detection of Novel Plant Capsid-Less Viruses: Papaya Umbra-like Viruses as Example
Use of Software for Primer Design
A Guide to Using FASTPCR Software for PCR, In Silico PCR, and Oligonucleotide Analysis
Primer Design for Newer PCR Approaches
Pyrosequencing Primer Design for Forensic Biology Applications
Phosphate-Methylated Oligonucleotides as a Novel Primer for PCR and RT-PCR