Pluschke Gerd, Röltgen Katharina (eds.) Mycobacterium ulcerans: Methods and Protocols

Humana Press, 2022. — 479 p. — (Methods in Molecular Biology 2387). — ISBN 978-1-0716-1779-3.This volume explores the latest techniques used to study Mycobacterium ulcerans, and more specifically M. ulcerans disease (Buruli ulcer). The chapters in this book are organized into three parts and cover methods for the detection of M. ulcerans and the analysis of host-pathogen interaction; the quantification and characterization of mycolactone, the macrolide toxin of M. ulcerans; and drug development against M. ulcerans. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mycobacterium ulcerans: Methods and Protocols is a valuable resource that helps scientists advance their research on Buruli ulcer, which is still an under-researched field in infection biology.Mycobacterium ulcerans Disease and Host Pathogen Interaction
Overview: Mycobacterium ulcerans Disease (Buruli Ulcer)
Immunohistochemistry: A Method to Analyze M. ulcerans Infected Skin Tissue
Primary Isolation of Mycobacterium ulcerans
Investigation of Mycobacterium ulcerans Glycan Interactions Using Glycan Array and Surface Plasmon Resonance
Purification and Characterization of Extracellular Vesicles from Mycobacterium ulcerans Culture
DNA Extraction from Clinical Specimens for the Direct Detection of Mycobacterium ulcerans by Real-Time PCR
Extraction of DNA from Environmental Samples for the Real-Time PCR Detection of Mycobacterium ulcerans
Detection of Mycobacterium ulcerans DNA Using Real-Time PCR
Determining Viability of M. ulcerans by 16S rRNA RT Reverse Transcriptase Real-Time PCR
Methods and Approaches for Buruli Ulcer Surveillance in Africa:​ Lessons Learnt and Future Directions
Purification and Quantification of Myolactone
Overview: Mycolactone, the Macrolide Toxin of Mycobacterium ulcerans
Conditions for Handling and Optimal Storage of Mycolactone
Mycolactone Purification from M. ulcerans Cultures and HPLC-Based Approaches for Mycolactone Quantification in Biological Samples
Detection of Mycolactone by Thin Layer Chromatography
Competitive ELISA for the Detection and Quantification of Mycobacterium ulcerans Mycolactone
Biochemical and Biological Assays of Mycolactone-Mediated Inhibition of Sec61
Drug Development Against Mycobacterium ulcerans
Overview: Development of Drugs Against Mycobacterium ulcerans
In Vitro Assessment of Drug Activity Against Mycobacterium ulcerans
Drug Efficacy Testing in the Mouse Footpad Model of Buruli Ulcer
Turbidity-Based MIC Assay and Characterization of Spontaneous Drug Resistant Mutants in Mycobacterium ulcerans
Determination of Bioenergetic Parameters in Mycobacterium ulcerans