Gnudi,Luigi, Long David A. (eds.) Diabetic Nephropathy: Methods and Protocols

Humana Press, 2020. — 337 p. — (Methods in Molecular Biology 2067). — ISBN 978-1-4939-9840-1.This book provides a toolkit of novel research approaches for investigators to study diabetic nephropathy, including critical experimental models from the fly to the fish, cells in culture, and in vivo mammalian approaches. The collection also explores powerful techniques to image the kidney, such as traditional histological techniques as well as electron, confocal, and two-photon microscopy, pathophysiology of the diabetic kidney, and gene editing and regenerative medicine. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Diabetic Nephropathy: Methods and Protocols seeks to foster new research directions and inspire ideas to enhance our understanding of diabetic nephropathy and to develop treatments for this condition.Diabetic Nephropathy: An Overview
Experimental Models to Study Diabetic Nephropathy
Modeling Podocyte Biology Using
A Technique for Studying Glomerular Filtration Integrity in the Zebrafish Pronephros
Modeling Human Diabetic Kidney Disease by Combining Hyperglycemia and Hypertension in a Transgenic Rodent Model
Isolating and Culturing Mouse Podocyte Cells to Study Diabetic Nephropathy
Techniques to Image the Diabetic Kidney
Histological Examination of the Diabetic Kidney
Sample Preparation and Stereological Methods for the Study of Glomerular Ultrastructure Using Electron Microscopy
Tissue Clearing and Deep Imaging of the Kidney Using Confocal and Two-Photon Microscopy
Assessing the Function and Pathophysiological Properties of the Diabetic Kidney
Transcutaneous Measurement of Glomerular Filtration Rate in Rodents
An In Vitro Method to Analyze Glucose Uptake in Podocytes
Glomerular Endothelial Cells: Assessment of Barrier Properties In Vitro
Methods to Detect Endoplasmic Reticulum Stress and Apoptosis in Diabetic Nephropathy
Isolating Urinary Extracellular Vesicles as Biomarkers for Diabetic Disease
Examining Cell-Cell Interactions in the Kidney Using AFM Single-Cell Force Spectroscopy
Transcriptome, Metabolic, and Proteomic Profiling of the Diabetic Kidney
Genetic Strategies to Understand Human Diabetic Nephropathy: Wet-Lab Approaches
Genetic Strategies to Understand Human Diabetic Nephropathy: In Silico Strategies for Molecular Data—Association Studies
Assessment of Urinary MicroRNAs by Quantitative Polymerase Chain Reaction in Diabetic Nephropathy Patients
Metabolomic and Proteomic Techniques for Establishing Biomarkers and Improving Our Understanding of Pathophysiology in Diabetic Nephropathy
New Approaches: Gene Editing and Stem Cells to Study Diabetic Kidney Disease
Formation of Mature Nephrons by Implantation of Human Pluripotent Stem Cell-Derived Progenitors into Mice
Generating Mutant Renal Cell Lines Using CRISPR Technologies