Zhao Feng, Leong Kam W. (eds.) Vascular Tissue Engineering: Methods and Protocols

Humana Press, 2022. — 560 p. — (Methods in Molecular Biology 2375). — ISBN 978-1-0716-1708-3.This volume explores the latest techniques used to study the field of tissue engineered vascular grafts (TEVGs). The chapters in this book cover a wide array of topics such as deriving vascular cells from monocytes and induced pluripotent stem cells; engineering vascular grafts using various biomaterials and stem cells, stem cell-derived, or primary vascular cells; biomaterial modification by anticoagulation molecules; vascular bioengineering technologies such as 3D bioprinting; and fabrication of TEVGs with different geometry and multiphase structures. This book also features protocols for grafting and evaluation of vascular grafts in animal models, vascular imaging in animals, and the quantification of blood vessel permeability. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Vascular Tissue Engineering: Methods and Protocols is a valuable resource for biomedical engineers, cell biologists, vascular surgeons, doctors, and nurses.Differentiating Human Pluripotent Stem Cells to Vascular Endothelial Cells for Regenerative Medicine, Tissue Engineering, and Disease Modeling
Generating Monocyte-Derived Endothelial-like Cells for Vascular Regeneration
Methods for Differentiating hiPSCs into Vascular Smooth Muscle Cells
On-Site Differentiation of Human Mesenchymal Stem Cells into Vascular Cells on Extracellular Matrix Scaffold Under Mechanical Stimulations for Vascular Tissue Engineering
End-Point Immobilization of Heparin on Electrospun Polycarbonate-Urethane Vascular Graft
Microfluidic Coaxial Bioprinting of Hollow, Standalone, and Perfusable Vascular Conduits
In Situ Fabrication and Perfusion of Tissue-Engineered Blood Vessel Microphysiologic​al System
Peritoneal Pre-conditioning Method for In Vivo Vascular Graft Maturation Utilizing a Porous Pouch
Fabrication of a Completely Biological and Anisotropic Human Mesenchymal Stem Cell-Based Vascular Graft
Engineering Vascular Grafts with Multiphase Structures
Fabrication of Small-Diameter Tubular Grafts for Vascular Tissue Engineering Applications Using Mulberry and Non-mulberry Silk Proteins
Fabrication and Evaluation of Tissue-Engineered Vascular Grafts with Hybrid Fibrous Structure
Controlling Pore Size of Electrospun Vascular Grafts by Electrospraying of Poly(Ethylene Oxide) Microparticles
Injectable Hydrogels for Vascular Tissue Engineering
Rabbit Surgery Protocol for End-to-End and End-to-Side Vascular Graft Anastomosis
Vascular Imaging in Small Animals Using Clinical Ultrasound Scanners
Vascular Graft Implantation Using a Bilateral End-to-Side Aortoiliac Preclinical Model
Quantification of In Vitro Blood-Brain Barrier Permeability
Subcellular Force Quantification of Endothelial Cells Using Silicone Pillar Arrays
Computational Assessment of Hemodynamics Vortices Within the Cerebral Vasculature Using Informational Entropy