Peng Xu, Zimmer Warren E. (eds.) Cardiovascular Development: Methods and Protocols

2nd edition. — Humana Press, 2021. — 164 p. — (Methods in Molecular Biology 2319). — ISBN 978-1-0716-1479-2.This second edition brings together a variety of method used in the field of cardiovascular development research. Chapters guide readers through protocols on lymphatic development, lymphatic staining techniques, lymphatic endothelial, tissue clearing techniques, and muscle cell isolation and culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cardiovascular Development: Methods and Protocols, Second Edition seeks to aid scientists in understanding new state-of-the-art techniques in the field of cardiovascular development research.Tissue Clearing and 3-D Visualization of Vasculature with the PEGASOS Method
Preparation and Identification of Cardiac Myofibrils from Whole Heart Samples
RNA-Sequencing and Transcriptome Analysis after Fluid Shear Stress Stimulation in Lymphatic Endothelial Cells
Preparation of Neonatal Rat Papillary Muscles for Contractile Studies
In Vitro Study of Permeability in Lymphatic Endothelial Cells Responding to Histamine
Optimizing the Differentiation of Cardiomyocytes from Human Induced Pluripotent-Derived Stem Cells
Analysis of Angiogenesis in Mouse Embryonic Dorsal Skin by Whole-Mount Fluorescent Staining
Isolation and Culture of Mouse Lymphatic Endothelial Cells from Lung Tissue
Laser-Induced Choroidal Neovascularization in Rats
Detecting Three-Dimensional Vascular Networks in the Mouse Embryonic Hindbrain
Visualizing Blood Vessel Development in Cultured Mouse Embryos Using Lightsheet Microscopy
Laser Capture Microdissection of Vascular Endothelial Cells from Frozen Heart Tissues
Visualization of Retinal Blood Vessels
Transient Transgenics: An Efficient Method to Identify Gene Regulatory Elements
Isolation of Lymphatic Muscle Cells (LMCs) from Rat Mesentery
Isolation of Adult Mouse Cardiomyocytes Using Langendorff Perfusion Apparatus
Analysis of Lymphatic Vessel Formation by Whole-Mount Immunofluorescence Staining