Dos Santos P.C. (Ed.) Fe-S Proteins: Methods and Protocols

Springer, 2021. — 352 p. — (Methods in Molecular Biology 2353). — ISBN 978-1-0716-1604-8.This volume explores current technologies used to investigate the formation, insertion, and function of metalloclusters associated with proteins. Chapters describe relevant topics about Fe-S cluster metabolism are explored through genetic, biochemical, spectroscopic methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Methods for genetic manipulation and Fe-S protein expression
Biochemical methods to probe steps involved in the synthesis of Fe-S clusters
Biophysical methods to study Fe-S clusters and reactions promoted by metalloenzymes