Malek Sami N. (ed.) Chronic Lymphocytic Leukemia: Methods and Protocols

Humana Press, 2019. — 279 p. — (Methods in Molecular Biology 1881). — ISBN 978-1-4939-8875-4.This book aims to provide scientists with tools and well-researched protocols to enable their research and to facilitate further progress in this common leukemia. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Chronic Lymphocytic Leukemia: Methods and Protocols aims to accelerate research on chronic lymphocytic leukemia and further improvements in patient outcomes.Ex-Vivo Signal Transduction Studies in Chronic Lymphocytic Leukemia
Ex Vivo Pharmacological Profiling in Chronic Lymphocytic Leukemia Cells
Culture and Harvest of CpG-Stimulated Peripheral Blood or Bone Marrow in Chronic Lymphocytic Leukemia
Analysis of Common Abnormalities Seen in Chronic Lymphocytic Leukemia Using Fluorescence In Situ Hybridization
Immunoglobulin Gene Analysis in Chronic Lymphocytic Leukemia
Detection and Functional Analysis of TP53 Mutations in CLL
The Development and Use of Scalable Systems for Studying Aberrant Splicing in SF3B1-Mutant CLL
Methods for Investigating VLA-4 (CD49d/CD29) Expression and Activation in Chronic Lymphocytic Leukemia and Its Clinical Applications
In Vitro Assay to Study CLL and Monocyte Interactions
Extracellular Flux Assays to Determine Oxidative Phosphorylation and Glycolysis in Chronic Lymphocytic Leukemia Cells
Measurement of Leukemic B-Cell Growth Kinetics in Patients with Chronic Lymphocytic Leukemia
Assays on DNA Damage and Repair in CLL
Method for Generating a Patient-Derived Xenograft Model of CLL
Immunological Methods to Study Monoclonal Antibody Activity in Chronic Lymphocytic Leukaemia
CRISPR/Cas9-Based Gene Dropout Screens
Gene Disruption Using CRISPR-Cas9 Technology
Minimal Residual Disease Quantification in Chronic Lymphocytic Leukemia: Clinical Significance and Flow Cytometric Methods
Rare Variant Quantitation Using Droplet Digital PCR
Methods for Measuring ctDNA in Lymphomas
Measurement of miRNAs in Chronic Lymphocytic Leukemia Patient Samples by Quantitative Reverse Transcription PCR
Bioinformatics Analysis of Whole Exome Sequencing Data
Genome Sequencing and Analysis Methods in Chronic Lymphocytic Leukemia
Characterization of Somatically-Acquired Copy Number Alterations in Chronic Lymphocytic Leukaemia Using Shallow Whole Genome Sequencing
High-Throughput Sequencing of the T-Cell Receptor Beta Chain Gene Repertoire in Chronic Lymphocytic Leukemia