Fortina P., Londin E., Park J.Y., Kricka L.J. (eds.) Acute Myeloid Leukemia: Methods and Protocols

Humana Press, 2017. — 279 p. — (Methods in Molecular Biology 1633). — ISBN 978-1-4939-7140-4.This volume describes up-to-date techniques used in understanding the molecular biology of acute myeloid leukemia (AML) and addressing advances in diagnosis, classification, prognostication, and therapeutic strategies to potentially impact overall patient survival. The chapters in this book cover topics such as: cytochemical staining, single-cell mass cytometry of AML and Leukemia stem/progenitor cells, microsphere-based assessment of DNA methylation for AML prognosis, a zebrafish model for evaluating the function of human leukemic gene IDH1 and its mutation, and the isolation of biologically active exosomes from plasma of patients with cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Acute Myeloid Leukemia: Methods and Protocols is a valuable resource for scientists and researchers to further their studies and advancements in the field of AML.Molecular Malfeasance Mediating Myeloid Malignancies: The Genetics of Acute Myeloid Leukemia
Cytochemical Staining
Immunohistochemistry in Acute Myeloid Leukemia
Immunophenotyping by Multiparameter Flow Cytometry
Single-Cell Mass Cytometry of Acute Myeloid Leukemia and Leukemia Stem/Progenitor Cells
Design and Application of Multiplex PCR Seq for the Detection of Somatic Mutations Associated with Myeloid Malignancies
AMLprofiler: A Diagnostic and Prognostic Microarray for Acute Myeloid Leukemia
Microsphere-Based Assessment of DNA Methylation for AML Prognosis
Genome-Wide Analysis of DNA Methylation in Hematopoietic Cells: DNA Methylation Analysis by WGBS
Detection and Quantification of Acute Myeloid Leukemia-Associated Fusion Transcripts
Targeted Next-Generation Sequencing of Acute Leukemia
Bioinformatics Data Analysis of Next-Generation Sequencing Data from Heterogeneous Tumor Samples
A Zebrafish Model for Evaluating the Function of Human Leukemic Gene IDH1 and Its Mutation
Measurement of Oncometabolites d-2-Hydroxyglutaric Acid and l-2-Hydroxyglutaric Acid
Nanofluidic Allele-Specific Digital PCR Method for Quantifying IDH1 and IDH2 Mutation Burden in Acute Myeloid Leukemia
Isolation of Biologically Active Exosomes from Plasma of Patients with Cancer
Generating and Expanding Autologous Chimeric Antigen Receptor T Cells from Patients with Acute Myeloid Leukemia