Stoddart Martin J. (ed.) Mammalian Cell Viability: Methods and Protocols

Humana Press, 2011. — 253 p. — (Methods in Molecular Biology 740). — ISBN 978-1-61779-107-9.Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required. In Mammalian Cell Viability: Methods and Protocols, experts in the field describe methods from the most basic which can be performed in any laboratory, to some which require specific pieces of equipment. Initially focusing on methods for monolayer and suspension cells, later chapters describe methods for determining viability within tissue sections and 3 dimensional culture systems. Finally, methods requiring highly specialized equipment are described in order to explain what is possible. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls. Practical and adaptable, Mammalian Cell Viability: Methods and Protocols serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incredibly important and influential field.Cell Viability Assays: Introduction
Cell Viability Analysis Using Trypan Blue: Manual and Automated Methods
Estimation of Cell Number Based on Metabolic Activity: The MTT Reduction Assay
WST-8 Analysis of Cell Viability During Osteogenesis of Human Mesenchymal Stem Cells
Assessment of Cell Proliferation with Resazurin-Based Fluorescent Dye
The xCELLigence System for Real-Time and Label-Free Monitoring of Cell Viability
Analysis of Tumor and Endothelial Cell Viability and Survival Using Sulforhodamine B and Clonogenic Assays
Annexin V/7-AAD Staining in Keratinocytes
Measurement of Caspase Activity: From Cell Populations to Individual Cells
Rapid Quantification of Cell Viability and Apoptosis in B-Cell Lymphoma Cultures Using Cyanine SYTO Probes
Multiplexing Cell Viability Assays
Cytotoxicity Testing: Measuring Viable Cells, Dead Cells, and Detecting Mechanism of Cell Death
Fluorescein Diacetate for Determination of Cell Viability in 3D Fibroblast–Collagen–GAG Constructs
Confocal Imaging Protocols for Live/Dead Staining in Three-Dimensional Carriers
Viability Assessment of Osteocytes Using Histological Lactate Dehydrogenase Activity Staining on Human Cancellous Bone Sections
Measuring Glutamate Receptor Activation-Induced Apoptotic Cell Death in Ischemic Rat Retina Using the TUNEL Assay
Exploiting the Liberation of Zn2+ to Measure Cell Viability
Noninvasive Bioluminescent Quantification of Viable Stem Cells in Engineered Constructs
Raman Micro-Spectroscopy as a Non-invasive Cell Viability Test
Closed Ampoule Isothermal Microcalorimetry for Continuous Real-Time Detection and Evaluation of Cultured Mammalian Cell Activity and Responses
Digital Image Processing of Live/Dead Staining