Postis V.L.G., Goldman A. (eds.) Biophysics of Membrane Proteins: Methods and Protocols

New York: Springer, 2020. — 322 p.This volume provides recent advances in the field of biophysics of membrane proteins. Chapters are divided into several parts: detailing biochemistry and functional analysis, experimental and theoretical structural determinations, membrane protein dynamics, and conformation studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Biophysics of Membrane Proteins: Methods and Protocols aims to provide comprehensive protocols with notes to help further the understanding of key membrane protein structure and function for students, academics, and industrial researchers.Membrane Protein Production and Purification from Escherichia coli and Sf9 Insect Cells
Quantifying the Interaction of Phosphite with ABC Transporters: MicroScale Thermophoresis and a Novel His-Tag Labeling Approach
Rationale for the Quantitative Reconstitution of Membrane Proteins into Proteoliposomes
Functional Characterization of SLC Transporters Using Solid Supported Membranes
Thermostability Assays: a Generic and Versatile Tool for Studying the Functional and Structural Properties of Membrane Proteins in Detergents
Direct Monitoring of GPCR Reconstitution and Ligand-Binding Activity by Plasmon Waveguide Resonance
Examining Membrane Proteins by Neutron Scattering
Solution X-Ray Scattering for Membrane Proteins
Interpreting SAXS/WAXS Data with Explicit-Solvent Simulations: A Practical Guide
Determining the Free Energies of Outer Membrane Proteins in Lipid Bilayers
Interrogating Membrane Protein Structure and Lipid Interactions by Native Mass Spectrometry
Determination of the Molecular Mass of Membrane Proteins Using Size-Exclusion Chromatography with Multiangle Laser Light Scattering (SEC-MALLS )
Dynamics of Membrane Proteins Monitored by Single-Molecule Fluorescence Across Multiple Timescales
Short-Range Distance Measurement by Transition Metal Ion FRET
PELDOR/DEER: An Electron Paramagnetic Resonance Method to Study Membrane Proteins in Lipid Bilayers