Vijay Rahul (ed.) MERS Coronavirus: Methods and Protocols

Humana Press, 2020. — 514 p. — (Methods in Molecular Biology 2099). — ISBN 978-1-0716-0211-9.This volume provides various techniques and methodologies currently used in the study of MERS-CoV. Chapters are divided into four parts detailing evolution and entry of MERS-coronavirus, genetic alteration and structural determination of MERS-coronavirus proteins, quantitation of virus and anti-viral factors, and mouse models for MERS -coronavirus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, MERS Coronavirus: Methods and Protocols aims to ensure successful results in the further study of this vital field.Evolution and Entry of MERS-Coronavirus
Studying Evolutionary Adaptation of MERS-CoV
Evaluating MERS-CoV Entry Pathways
Biochemical Characterization​ of Middle East Respiratory Syndrome Coronavirus Spike Protein Proteolytic Processing
Crystallization and Structural Determination of the Receptor-Binding Domain of MERS-CoV Spike Glycoprotein
Genetic Alteration and Structural Determination of MERS-Coronavirus Proteins
Bacterial Artificial Chromosome-Based Lambda Red Recombination with the I-SceI Homing Endonuclease for Genetic Alteration of MERS-CoV
Deducing the Crystal Structure of MERS-CoV Helicase
Quantitation of Virus and Anti-Viral Factors
Antigen Capture Enzyme-Linked Immunosorbent Assay for Detecting Middle East Respiratory Syndrome Coronavirus in Humans
Quantification of the Middle East Respiratory Syndrome-Coronavirus RNA in Tissues by Quantitative Real-Time RT-PCR
Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralizat​ion Assay
Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera
Qualitative and Quantitative Determination of MERS-CoV S1-Specific Antibodies Using ELISA
Mouse Models for MERS–Coronavirus
Genetically Engineering a Susceptible Mouse Model for MERS-CoV-Induced Acute Respiratory Distress Syndrome
Development of a Mouse-Adapted MERS Coronavirus
Metabolite, Protein, and Lipid Extraction (MPLEx):​ A Method that Simultaneously Inactivates Middle East Respiratory Syndrome Coronavirus and Allows Analysis of Multiple Host Cell Components Following Infection
Evaluation of Activation and Inflammatory Activity of Myeloid Cells During Pathogenic Human Coronavirus Infection
Histopathologic Evaluation and Scoring of Viral Lung Infection