Ribatti Domenico (ed.) Vascular Morphogenesis: Methods and Protocols

2nd edition. — Humana Press, 2021. — 297 p. — (Methods in Molecular Biology 2206). — ISBN 978-1-0716-0915-6.This detailed new edition aims to provide a range of methods and protocols for studying vascular morphogenesis in vivo and in vitro to reflect up-to-date advances in the field. Of note, the book explores endothelial cell signaling is currently understood to promote fundamental cues for cell fate specification, embryo patterning, organ differentiation, and postnatal tissue remodeling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and timely, Vascular Morphogenesis: Methods and Protocols, Second Edition will serve as an ideal guide to this area of study for a broad audience amongst cell biologists, anatomists, pathologists, and physiologists.Historical Overview of In Vivo and In Vitro Angiogenesis Assays
The Fundamental Contribution of Judah Folkman in the Setting of Angiogenesis Assays
Identification of Endothelial Cells and Their Progenitors
In Vitro Coculture Assays of Angiogenesis
Endothelial Cells: Co-culture Spheroids
Microfluidic Device Setting by Coculturing Endothelial Cells and Mesenchymal Stem Cells
Spatial Statistics-Based Image Analysis Methods for the Study of Vascular Morphogenesis
Studying Angiogenesis in the Rabbit Corneal Pocket Assay
Avians as a Model System of Vascular Development
Dynamic Imaging of Mouse Embryos and Cardiac Development in Static Culture
High-Resolution Confocal Imaging of Pericytes in Human Fetal Brain Microvessels
Quantification of Tumor Vasculature by Analysis of Amount and Spatial Dispersion of Caliber-Classified Vessels
A Xenograft Model for Venous Malformation
In Vivo Vascular Network Forming Assay
Assessment of Vascular Patterning in the Zebrafish
By the Skin of Your Teeth: A Subcutaneous Mouse Model to Study Pulp Regeneration