Mo R. Ebrahimkhani., Joshua Hislop Programmed Morphogenesis

Springer, 2021. — 286 p. — (Methods in Molecular Biology 2258). — ISBN-13. 978-1-0716-1174-6
This detailed book explores techniques for understanding and engineering programs that naturally control and drive formation of tissues and organs in order to open powerful opportunities to produce physiologically relevant tissues of interest, generate models to study human disease, and set the path for the manufacturing of advanced tissue and organs. Beginning with chapters to help understand signaling events and patterns in morphogenetic systems, the volume continues by covering programming signaling events and patterns to drive morphogenesis, techniques for engineering organoids, tissue barriers, and disease models, as well as in vivo therapeutic applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Programmed Morphogenesis: Methods and Protocols aims not only to communicate knowledge but also to inspire approaches to new challenges and to empower readers with the capability to approach those challenges.TASBE Image Analytics: A Processing Pipeline for Quantifying Cell Organization from Fluorescent Microscopy
Neighborhood Impact Factor to Study Cell-Fate Decision-Making in Cellular Communities
A Quantitative Lineage-Tracing Approach to Understand Morphogenesis in Gut
Reconstitution of Morphogen Signaling Gradients in Cultured Cells
Engineering Shape-Controlled Microtissues on Compliant Hydrogels with Tunable Rigidity and Extracellular Matrix Ligands
Engineering Biophysical Cues for Controlled 3D Differentiation of Endoderm Derivatives
Rewiring Endogenous Bioelectric Circuits in the Xenopus laevis Embryo Model
Engineering the Spatiotemporal Mosaic Self-Patterning of Pluripotent Stem Cells
Fate-Patterning of 2D Gastruloids and Ectodermal Colonies Using Micropatterned Human Pluripotent Stem Cells
Gastruloids: Embryonic Organoids from Mouse Embryonic Stem Cells to Study Patterning and Development in Early Mammalian Embryos
A Synergistic Engineering Approach to Build Human Brain Spheroids
Directed Differentiation of Human Pluripotent Stem Cells for the Generation of High-Order Kidney Organoids
Methods for Controlled Induction of Singular Rosette Cytoarchitecture Within Human Pluripotent Stem Cell-Derived Neural Multicellular Assemblies
3D Self-Organized Human Blood–Brain Barrier in a Microfluidic Chip
Modeling the Complexity of the Metastatic Niche Ex Vivo
Fabrication Method of a High-Density Co-Culture Tumor–Stroma Platform to Study Cancer Progression
A Method for Organoid Transplantation and Whole-Mount Visualization of Post-Engraftment Vascularization
High-Throughput Production of Platelet-Like Particles