Azevedo Nuno F., Almeida Carina (eds.) Fluorescence In-Situ Hybridization (FISH) for Microbial Cells: Methods and Concepts

Humana Press, 2021. — 338 p. — (Methods in Molecular Biology 2246). — ISBN 978-1-0716-1114-2.This volume provides a comprehensive review of concepts and protocols related to fluorescence in situ hybridization (FISH) applied to microbial cells. Chapters will serve guide for the design of new probes and the development of novel FISH-based protocols. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Fluorescence In Situ Hybridization (FISH) for Microbial Cells: Methods and Concepts aims to ensure successful results in the further study of this vital field.An Introduction to Fluorescence in situ Hybridization in Microorganisms
FISH Variants
Bioinformatic Tools and Guidelines for the Design of Fluorescence In Situ Hybridization Probes
FISH in Suspension or in Adherent Cells
Application of Nucleic Acid Mimics in Fluorescence In Situ Hybridization
Delivery of Oligonucleotides into Bacteria by Fusogenic Liposomes
Characterization of Social Interactions and Spatial Arrangement of Individual Bacteria in MultiStrain or Multispecies Biofilm Systems Using Nucleic Acid Mimics-Fluorescence In Situ Hybridization
Leaf-FISH: In Situ Hybridization Method for Visualizing Bacterial Taxa on Plant Surfaces
CAtalyzed Reporter Deposition Fluorescence In Situ Hybridization (CARD-FISH) for Complex Environmental Samples
Fluorescence In Situ Hybridization with Quantum Dot Labels in E. coli Cells
Monitoring Bacteriophage Infection on Bacterial Cells Using FISH
Linking Microbes to Their Genes at Single Cell Level with Direct-geneFISH
Assigning Function to Phylogeny: FISH-nanoSIMS
Assigning Function to Phylogeny: MAR-FISH
Counting mRNA Copies in Intact Bacterial Cells by Fluctuation Localization Imaging-Based Fluorescence In Situ Hybridization (fliFISH)
ntegration of FISH and Microfluidics
Flow-FISH Using Nucleic Acid Mimic Probes for the Detection of Bacteria
FISH in Food Samples
Extraction of Microbial Cells from Environmental Samples for FISH Approaches
Quality Control in Diagnostic Fluorescence In Situ Hybridization (FISH) in Microbiology
Computational Resources and Strategies to Construct Single-Molecule Models of FISH