Caugant D.A. (Ed.) Molecular Epidemiology of Microorganisms: Methods and Protocols

Springer, 2009. — 211 p. — (Methods in Molecular Biology 551). — ISBN 978-1-60327-998-7.The development of molecular genotyping methods has revolutionized the possibility for classification of microorganisms at the sub-species level. This investigation of species diversity is crucial for deciding the molecular relatedness of isolates for epidemiological studies. In Molecular Epidemiology of Microorganisms: Methods and Protocols, readers will find readily reproducible methods for determining the molecular epidemiology of microorganisms, all of which provide effective discrimination of unrelated strains. This volume covers a wide range of techniques which can be easily applied to the investigation of transmissible diseases, directing researchers towards the most effective methods based on the particular information to be obtained. Describing both traditional and novel techniques, expert researchers present a series of methods-based chapters with applications to some of the most important microbes. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Molecular Epidemiology of Microorganisms: Methods and Protocols provides state-of-the-art techniques which will prove to be critical in unraveling the routes of spread of pathogens for humans, animals, and plants.Microbial Molecular Epidemiology: An Overview
Multilocus Enzyme Electrophoresis for Parasites and Other Pathogens
Plasmid Replicon Typing
The Application of Randomly Amplified DNA Analysis in the Molecular Epidemiology of Microorganisms
Use of Repetitive Element Palindromic PCR (rep-PCR) for the Epidemiologic Discrimination of Foodborne Pathogens
Pulsed-Field Gel Electrophoresis for Molecular Epidemiology of Food Pathogens
Molecular Genotyping of Microbes by Multilocus PCR and Mass Spectrometry: A New Tool for Hospital Infection Control and Public Health Surveillance
Amplified Fragment Length Polymorphism Analysis
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) for the Genotyping of Bacterial Pathogens
Spoligotyping for Molecular Epidemiology of the Mycobacterium tuberculosis Complex
Multilocus Sequence Typing
Multiple Locus Variable Number of Tandem Repeats Analysis
Comparison of Molecular Typing Methods Applied to Clostridium difficile
Genotyping of Mycobacterium tuberculosis Clinical Isolates Using IS6110-Based Restriction Fragment Length Polymorphism Analysis
spa Typing for Epidemiological Surveillance of Staphylococcus aureus
Sequencing of Viral Genes
Full Sequencing of Viral Genomes: Practical Strategies Used for the Amplification and Characterization of Foot-and-Mouth Disease Virus
Bacterial Genome Sequencing
DNA Microarray for Molecular Epidemiology of Salmonella
Methods for Data Analysis
Internet-Based Sequence-Typing Databases for Bacterial Molecular Epidemiology