Pfeifer Blaine A., Hill Andrew (eds.) Vaccine Delivery Technology: Methods and Protocols

Humana Press, 2021. — 595 p. — (Methods in Molecular Biology 2183). — ISBN 978-1-0716-0794-7.This volume discusses the vaccine development process and the role delivery concepts contribute to a global goal of effective health outcomes. The chapters in this book cover a wide range of topics such as antigen discovery methods; genetic and protein antigen preparation; preparation of viral vaccines as VLPs; viral and non-viral gene delivery; needle-less or non-invasive delivery technology; vaccine storage; and vaccine administration and assessment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics; lists of the necessary materials and reagents; step-by-step, readily reproducible laboratory protocols; and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Vaccine Delivery Technology: Methods and Protocols is a valuable resource for both novice and expert researchers, in and outside the field, who would like to gain insight into the impactful field of vaccines.Vaccine Delivery and Immune Response Basics
Isolating Pathogen-Specific Human Monoclonal Antibodies (hmAbs) Using Bacterial Whole Cells as Molecular Probes
Use of Chlamydial Elementary Bodies as Probes to Isolate Pathogen-Specific Human Monoclonal Antibodies
Computational Antigen Discovery for Eukaryotic Pathogens Using Vacceed
Antigen Discovery in Bacterial Panproteomes
Alphavirus-Based Antigen Preparation
Production of Chimeric Hepatitis B Virus Surface Antigens in Mammalian Cells
Bioreactor-Based Antigen Production Process Using the Baculovirus Expression Vector System
High-Throughput Process Development for the Chromatographic Purification of Viral Antigens
Production of Zika Virus Virus-Like Particles
Biosynthesis of Glycoconjugate Virus-like Particles (VLPs)
Upstream and Downstream Processes for Viral Nanoplexes as Vaccines
Whole-Cell Vaccine Preparation: Options and Perspectives
O-Antigen Extraction, Purification, and Chemical Conjugation to a Carrier Protein
Oligosaccharide Antigen Conjugation to Carrier Proteins to Formulate Glycoconjugate Vaccines
Exploitation of Capsule Polymerases for Enzymatic Synthesis of Polysaccharide Antigens Used in Glycoconjugate Vaccines
Attenuation Methods for Live Vaccines
Surface Modification of Adenovirus Vector to Improve Immunogenicity and Tropism
Production of Baculovirus and Stem Cells for Baculovirus-Mediated Gene Transfer into Human Mesenchymal Stem Cells
Lipofection-Based Delivery of DNA Vaccines
Flavivirus DNA Vaccine Design and Adjuvant Selection
Vaccine Delivery with a Detoxified Bacterial Toxin
Needleless or Noninvasive Delivery Technology
Lactic Acid Bacteria as Delivery Vehicle for Therapeutics Applications
A Hybrid Biological–Biomaterial Vector for Antigen Delivery
Liposomal Dual Delivery of Both Polysaccharide and Protein Antigens
Thin-Film Freeze-Drying Is a Viable Method to Convert Vaccines Containing Aluminum Salts from Liquid to Dry Powder
Application of Cryogenic Transmission Electron Microscopy for Evaluation of Vaccine Delivery Carriers
Methods to Evaluate Immune Cell Recruitment and Cellular Uptake and Distribution of Antigen Following Intramuscular Administration of Vaccine to Mice
The Quantification of Antigen-Specific T Cells by IFN-γ ELISpot
Measurement of Antigen-Specific IgG Titers by Direct ELISA
A Method to Evaluate In Vivo CD8+ T Cell Cytotoxicity in a Murine Model
Testing Anti-Pneumococcal Antibody Function Using Bacteria and Primary Neutrophils
Viral Infectivity Quantification and Neutralization Assays Using Laser Force Cytology