Kolpashchikov Dmitry M., Gerasimova Yulia V. (eds.) Nucleic Acid Detection: Methods and Protocols

Humana Press, 2013. — 312 p. — (Methods in Molecular Biology 1039). — ISBN 978-1-62703-534-7.In Nucleic Acid Chemistry: Methods and Protocols, expert researches in the field detail techniques and approaches for the detection of DNA and RNA. These techniques include the recovery of trace amounts of DNA for amplification and analysis, new qPCR chemistries, new application of isothermal amplification techniques, assays with visual or electric signals for point-of-care diagnostics, improvement of fluorescent in situ hybridization, and new signal amplification techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Nucleic Acid Chemistry: Methods and Protocols seeks to aid scientists in the further study of detection for DNA and RNA.Advances in Sample Preparation
“Getting Blood from a Stone”: Ultrasensitive Forensic DNA Profiling of Microscopic Bio-Particles Recovered from “Touch DNA” Evidence
Detecting MicroRNA in Human Cancer Tissues with Fluorescence In Situ Hybridization
New Real-Time and Instantaneous Assays
MNAzyme qPCR: A Superior Tool for Multiplex qPCR
A New, Multiplex, Quantitative Real-Time Polymerase Chain Reaction System for Nucleic Acid Detection and Quantification
Detection of SNP-Containing Human DNA Sequences Using a Split Sensor with a Universal Molecular Beacon Reporter
Isothermal Amplifi cation Methods for Nucleic Acid Detection
SNP Analysis Using a Molecular Beacon-Based Operating Cooperatively (OC) Sensor
Detection of rpoB Gene Mutations Using Helicase-Dependent Amplification
Rapid Detection of Brucella spp. Using Loop-Mediated Isothermal Amplification (LAMP)
Loop-Mediated Isothermal Amplification Method for a Differential Identification of Human Taenia Tapeworms
Detection of Mutation by Allele-Specific Loop-Mediated Isothermal Amplification (AS-LAMP)
Signal Amplifi cation Approach
DNA Detection by Cascade Enzymatic Signal Amplification
Non-fluorescent Detection Formats
Visual DNA Detection and SNP Genotyping Using Asymmetric PCR and Split DNA Enzymes
Detection of Single-Stranded Nucleic Acids via Colorimetric Means, Using G-Quadruplex Probes
Lateral Flow Biosensors for the Detection of Nucleic Acid
Electrochemiluminescence Detection of c-Myc mRNA in Breast Cancer Cells on a Wireless Bipolar Electrode
High-Throughput Label-Free Detection of DNA Hybridization and Mismatch Discrimination Using Interferometric Reflectance Imaging Sensor
Graphene–PAMAM Dendrimer–Gold Nanoparticle Composite for Electrochemical DNA Hybridization Detection
Advances in FISH
Application of PNA Openers for Fluorescence-Based Detection of Bacterial DNA
DNA Probes for FISH Analysis of C-Negative Regions in Human Chromosomes
Detection of MicroRNA
Quantitative Analysis of MicroRNA in Blood Serum with Protein-Facilitated Affinity Capillary Electrophoresis
High-Throughput Functional MicroRNA Profiling Using Recombinant AAV-Based MicroRNA Sensor Arrays
The Use of Molecular Beacons to Detect and Quantify MicroRNA
RNA Imaging in Live Cells
Sequence-Specific Imaging of Influenza A mRNA in Living Infected Cells Using Fluorescent FIT–PNA
Application of Caged Fluorescent Nucleotides to Live-Cell RNA Imaging