Mao Chai-An (ed.) Retinal Development: Methods and Protocols

Humana Press, 2020. — 241 p. — (Methods in Molecular Biology 2092). — ISBN 978-1-0716-0174-7.This volume details commonly used molecular and cellular techniques and specialized methodologies for studying retina neuronal subtypes and electrophysiology. Chapters describe techniques for anatomical studies of retinal ganglion cell morphology, gap-junction-mediated neuronal connection, multi-electrode array recording on mouse retinas, and paired recording to study the electrical coupling between photoreceptors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Retinal Development: Methods and Protocols aims to provide readers with a set of practical experimental tools to study retinal development, regeneration, and function of mature retinal neurons. Many of the protocols and strategies described in one organism can be easily adapted to applications in different model systems.Investigating Neurogenesis in Birds
Studying In Vivo Retinal Progenitor Cell Proliferation in Xenopus laevis
Three-Dimensional Culture of Mouse Eyecups
Live Imaging of Mouse Retinal Slices
Retinal Strip Culture for Studying Ganglion Cell Axon Growth
Multiple Approaches for Enhancing Neural Activity to Promote Neurite Outgrowth of Retinal Explants
Adeno-Associated Virus as Gene Delivery Vehicle into the Retina
Transposon-Mediated Stable Suppression of Gene Expression in the Developing Chick Retina
A Simple Guide for Generating BAC Transgenic Animals for Retinal Research
Identification and Characterization of Cis-Regulatory Elements for Photoreceptor-Type-Specific Transcription in ZebraFish
Ultrasensitive RNAscope In Situ Hybridization System on Embryonic and Adult Mouse Retinas
Single-Cell Capture, RNA-seq, and Transcriptome Analysis from the Neural Retina
Genetically Directed Sparse Labeling System for Anatomical Studies of Retinal Ganglion Cells
Intracellular Dye Microinjection in Retinal Morphological and Communication Studies
Multielectrode Array Recording of Mouse Retinas Transplanted with Stem Cell-Derived Retinal Sheets
Paired Recording to Study Electrical Coupling Between Photoreceptors in Mouse Retina