Sprecher Simon G. (ed.) Brain Development: Methods and Protocols

2nd edition. — Humana Press, 2020. — 520 p. — (Methods in Molecular Biology 2047). — ISBN 978-1-4939-9731-2.This book provides a thorough introduction to widely used techniques for the study of the intersection between developmental biology and neuroscience, an exceptional area to address and investigate impacting biological questions. The fully updated volume examines cutting-edge techniques on a representative range of animals, including widely used genetic model systems, such as the fruit fly, zebra fish, chicken, and mouse, as well as non-canonical experimental systems opened up through the advent of genome editing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Brain Development: Methods and Protocols, Second Edition is an ideal guide for researchers interested in utilizing recent technical advances in molecular genetics for the study of the brain.Invertebrate Models
Combining BrdU-Labeling to Detection of Neuronal Markers to Monitor Adult Neurogenesis in Hydra
Reverse Genetic Approaches to Investigate the Neurobiology of the Cnidarian Sea Anemone Nematostella vectensis
Generating Transgenic Reporter Lines for Studying Nervous System Development in the Cnidarian Nematostella vectensis
Immunostaining and In Situ Hybridization of the Developing Acoel Nervous System
Immunostaining of the Embryonic and Larval Drosophila Brain
Nonfluorescent RNA In Situ Hybridization Combined with Antibody Staining to Visualize Multiple Gene Expression Patterns in the Embryonic Brain of Drosophila
Analysis of Complete Neuroblast Cell Lineages in the Drosophila Embryonic Brain via DiI Labeling
Flybow to Dissect Circuit Assembly in the Drosophila Brain: An Update
Live Cell Imaging of Neural Stem Cells in the Drosophila Larval Brain
CRISPR/Cas9 Genome Editing to Study Nervous System Development in Drosophila
The Red Flour Beetle as Model for Comparative Neural Development: Genome Editing to Mark Neural Cells in Tribolium Brain Development
A Protocol for Double Fluorescent In Situ Hybridization and Immunohistochemistry for the Study of Embryonic Brain Development in Tribolium castaneum
Immunohistochemistry and Fluorescent Whole Mount RNA In Situ Hybridization in Larval and Adult Brains of Tribolium
X-Ray Microscopy of the Larval Crustacean Brain
Immunolocalization of Neurotransmitters and Neuromodulators in the Developing Crayfish Brain
Immunostainings in Nervous System Development of the Nematode C. elegans
Methods in Brain Development of Molluscs
A Simple Method to Identify Ascidian Brain Lineage Cells at Neural Plate Stages Following In Situ Hybridization
Spawning Induction and Embryo Micromanipulation Protocols in the Amphioxus Branchiostoma lanceolatum
Vertebrate Models
In Situ Hybridization and Immunostaining of Xenopus Brain
Morpholino Studies in Xenopus Brain Development
Sensitive Multiplexed Fluorescent In Situ Hybridization Using Enhanced Tyramide Signal Amplification and Its Combination with Immunofluorescent Protein Visualization in Zebrafish
Live Morphometric Classification of Sensory Neurons in Larval Zebrafish
Immunohistochemistry and In Situ Hybridization in the Developing Chicken Brain
Gene Silencing in Chicken Brain Development
Transplantation of Neural Tissue: Quail–Chick Chimeras
Immunohistochemistry and RNA In Situ Hybridization in Mouse Brain Development
The Cre/Lox System to Assess the Development of the Mouse Brain
In Utero Electroporation to Study Mouse Brain Development