Martina Marzia, Taverna Stefano (eds.) Patch-Clamp: Methods and Protocols

2nd edition. — Humana Press, 2014. — 400 p. — (Methods in Molecular Biology 1183). — ISBN: 978-1-4939-1095-3.Since its inception, patch-clamp has continued to be widely considered the gold standard method to record ion channel activity. Patch-Clamp Methods and Protocols, Second Edition, provides a comprehensive collection of new techniques for the development of automated, high-throughput screening systems for pharmacological evaluation, the use of various patch-clamp configurations together with novel molecular biological and imaging methodologies and enhanced stimulation protocols and perfusion systems. Divided into sections on pharmacology, physiology and biophysics, the chapters cover methods to generate more physiologically relevant conditions for drug application and screening technologies, recently developed applications such as optogenetic stimulation, advances in whole-cell recordings in freely-moving animals and novel technologies to create custom microelectrodes designed for reducing the access resistance and improving the rate of molecular diffusion. Patch-clamp is an indispensable technique for conducting pharmacological, physiological and biophysical research aimed at understanding crucial aspects of cellular and network function. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Methods and Protocols, Second Edition will provide a useful technical and methodological guide to diverse audiences of electrophysiologists, from students to experienced investigators.History of Electrophysiology and the Patch Clamp
Pharmacology
Whole-Cell Patch-Clamp Analysis of Recombinant NMDA Receptor Pharmacology Using Brief Glutamate Applications
Patch Clamp Combined with Voltage/Concentration Clamp to Determine the Kinetics and Voltage Dependency of N-Methyl-d-aspartate (NMDA) Receptor Open Channel Blockers
State-of-the-Art Automated Patch Clamp: Heat Activation, Action Potentials, and High Throughput in Ion Channel Screening
Industrializing Electrophysiology: HT Automated Patch Clamp on SyncroPatch 96 Using Instant Frozen Cells
Planar Patch Clamp for Neuronal Networks—Considerations and Future Perspectives
Physiology
Methods for the Study of Synaptic Receptor Functional Properties
Single-Cell RT-PCR, a Technique to Decipher the Electrical, Anatomical, and Genetic Determinants of Neuronal Diversity
Whole-Cell Voltage Clamp on Skeletal Muscle Fibers with the Silicone-Clamp Technique
Multiphoton Imaging Approaches for Studying Striatal Dendritic Excitability
Investigation of Synaptic Microcircuits Using Patch-Clamp Paired Recordings in Acute Brain Slices
Outside-Out “Sniffer-Patch” Clamp Technique for In Situ Measures of Neurotransmitter Release
A Cost-Effective Method for Preparing, Maintaining, and Transfecting Neurons in Organotypic Slices
Acute Brain Slice Methods for Adult and Aging Animals: Application of Targeted Patch Clamp Analysis and Optogenetics
Synaptic Connectivity in Engineered Neuronal Networks
Modeling of Action Potential Generation in NG108-15 Cells
Whole-Cell Patch-Clamp Recordings in Freely Moving Animals
Biophysics
Pressure-Polished Borosilicate Pipettes are “Universal Sealer” Yielding Low Access Resistance and Efficient Intracellular Perfusion
Current Recordings at the Single Channel Level in Adult Mammalian Isolated Cardiomyocytes
Dynamic Clamp as a Tool to Study the Functional Effects of Individual Membrane Currents
Dynamic Clamp in Cardiac and Neuronal Systems Using RTXI
Ion Selectivity of Pore-Forming Peptides and Ion Channels Measured in Xenopus Oocytes
Principles of Single-Channel Kinetic Analysis