Dallas Mark, Bell Damian (eds.) Patch Clamp Electrophysiology: Methods and Protocols

Humana Press, 2021. — 333 p. — (Methods in Molecular Biology 2188). — ISBN: 978-1-0716-0817-3.This volume describes a range of standard and novel methodological approaches used to probe ion channel function across different modalities. Chapters guide readers through methods and protocols from an introduction to the decades old patch clamp method for the ion channel neophyte to more complex, recent protocol advances, such as optogenetics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Patch Clamp Electrophysiology: Methods and Protocols aims to be a reference guide for current and future ion channel physiologists.An Introduction to Patch Clamp Recording
Patch Clamp Technology in the Twenty-First Century
Heterologous Expression of Ion Channels in Mammalian Cell Lines
Electrophysiology on Channel-Forming Proteins in Artificial Lipid Bilayers: Next-Generation Instrumentation for Multiple Recordings in Parallel
Perforated Whole-Cell Recordings in Automated Patch Clamp Electrophysiology
Multielectrode Arrays
Utilising Automated Electrophysiological Platforms in Epilepsy Research
Dynamic Clamp on a Windows PC
Combining Whole-Cell Patch-Clamp Recordings with Single-Cell RNA Sequencing
Bioluminescence Methodology for Ion Channel Studies
Nucleated, Outside-Out, Somatic, Macropatch Recordings in Native Neurons
Preparation of Rat Organotypic Hippocampal Slice Cultures Using the Membrane-Interface Method
In Vivo Patch-Clamp Studies
In Vivo Optogenetics with Stimulus Calibration
Single or Double Patch-Clamp Recordings In Ex Vivo Slice Preparation: Functional Connectivity, Synapse Dynamics, and Optogenetics
Optogenetics and Optical Tools in Automated Patch Clamping