Lucas Alexandra R. (ed.) Viruses as Therapeutics: Methods and Protocols

Humana Press, 2021. — 297 p. — (Methods in Molecular Biology 2225). — ISBN: 978-1-0716-1011-4.This detailed volume explores methods and techniques reflecting how viruses can be a beneficial force for all levels of life as well as the mammalian microbiota. Beginning with methods for the development and study of viruses, the book continues with current techniques and approaches used to develop new treatments from virus-derived vaccines to vectors and proteins, such as a range of models and methods designed to measure therapeutic efficacy of virus-derived biologics and therapeutic proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Viruses as Therapeutics: Methods and Protocols is an ideal guide for researchers working to further understand how viruses can benefit lifeforms and even prevent damage from other viruses.Viruses for Landscape-Scale Therapy: Biological Control of Rabbits in Australia
Development and Expression of Subunit Vaccines Against Viruses in Plants
Development of Antiviral Vaccine Utilizing Self-Destructing Salmonella for Antigen and DNA Vaccine Delivery
Methods for the Construction of Recombinant Oncolytic Myxoma Viruses
Molecular Design and Production of AAV Viral Vectors for Gene Therapy
Murine Model of Thermal Burn Injury for Evaluating Protein Therapeutics Derived from Viruses
Deriving Immune-Modulating Peptides from Viral Serine Protease Inhibitors (Serpins)
Methods for Crystallization and Structural Determination of M-T7 Protein from Myxoma Virus
Microscopic Analysis of Viral Infection
Metabolomics Analysis of Viral Therapeutics
Detecting Necroptosis in Virus-Infected Cells
Topical Application of Virus-Derived Immunomodulating Proteins and Peptides to Promote Wound Healing in Mouse Models
Neurologic and Histologic Tests Used to Measure Neuroprotective Effectiveness of Virus-Derived Immune-Modulating Proteins
Preclinical Testing of Viral Therapeutic Efficacy in Pristane-Induced Lupus Nephritis and Diffuse Alveolar Hemorrhage Mouse Models
Kidney Subcapsular Allograft Transplants as a Model to Test Virus-Derived Chemokine-Modulating Proteins as Therapeutics
A Mouse Model of Acute Liver Injury by Warm, Partial Ischemia-Reperfusion for Testing the Efficacy of Virus-Derived Therapeutics