Baron Margaret H. (ed.) Developmental Hematopoiesis: Methods and Protocols

Humana Press, 2005. — 481 p. — (Methods in Molecular Medicine 105). — ISBN: 978-1-58829-296-4.Over the past decade technical and conceptual breakthroughs, cross-species comparisons, and multidisciplinary approaches have led to a virtual revolution in developmental biology. In Developmental Hematopoiesis: Methods and Protocols, a panel of expert investigators critically surveys an array of methods available to study hematopoiesis and describes in detail the powerful techniques they have established to investigate embryonic, fetal, and (to a lesser extent) postnatal blood development. The authors apply their approaches to invertebrate (fruit flies) as well as vertebrate (frog, fish, mouse, chick, and human) organisms. These state-of-the-art techniques range from the genetic, molecular, and cellular, to cell and embryo explant culture and whole animals, including in vivo imaging. Bioinformatic and functional genomic approaches for studying stem cells and their supportive stromal cells are also discussed. The protocols follow the successful Methods in Molecular Medicine series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and highly practical, Developmental Hematopoiesis: Methods and Protocols offers novice and seasoned researchers alike the critical tools needed to gain a fundamental understanding of stem cell biology-examining not only how hematopoietic stem cells form, but also how they differ in the embryo, the fetus, and the adult.Gene Targeting and Transgenic Strategies for the Analysis of Hematopoietic Development in the Mouse
Inducible Transgene Expression in Mouse Stem Cells
Quantitative Trait Analysis in the Investigation of Function and Aging of Hematopoietic Stem Cells
Flow Cytometric Analysis of Hematopoietic Development
Reconstitution of Hematopoiesis Following Intrauterine Transplantation of Stem Cells
Reconstitution of Hematopoiesis Following Transplantation Into Neonatal Mice
Hemocyte Development During Drosophila Embryogenesis
Tracking and Programming Early Hematopoietic Cells in Xenopus Embryos
Fate Mapping Hematopoietic Lineages in the Xenopus Embryo
Analyses of Immune Responses to Ontogeny-Specific Antigens Using an Inbred Strain of Xenopus laevis (J Strain)
Use of Flow Cytometry and Combined DNA Surface Staining for Analysis of Hematopoietic Development in the Xenopus Embryo
Analysis of Hematopoietic Development in the Zebrafish
Imaging Early Macrophage Differentiation, Migration, and Behaviors in Live Zebrafish Embryos
In Vivo Methods to Analyze Cell Origins, Migrations, Homing, and Interactions in the Blood, Vascular, and Immune Systems of the Avian and Mammalian Embryo
Mouse Embryonic Explant Culture System for Analysis of Hematopoietic and Vascular Development
Hematopoietic Stem Cell Enrichment From the AGM Region of the Mouse Embryo
Hematopoietic Stem Cell Development During Mouse Embryogenesis
Analysis of Hematopoietic Progenitors in the Mouse Embryo
In Vivo and In Vitro Assays of Thymic Organogenesis
Retroviral Transduction in Fetal Thymic Organ Culture
Expansion and Differentiation of Immature Mouse and Human Hematopoietic Progenitors
A New Clonal Assay System for Lymphoid and Myeloid Lineages
Hematopoietic and Endothelial Development of Mouse Embryonic Stem Cells in Culture
Analysis of the Vascular Potential of Hematopoietic Stem Cells
Dynamic In Vivo Imaging of Mammalian Hematovascular Development Using Whole Embryo Culture
Fluorescent Protein-Cell Labeling and Its Application in Time-Lapse Analysis of Hematopoietic Differentiation
Analysis of Hematopoietic Development During Human Embryonic Ontogenesis
Hematopoietic Development of Human Embryonic Stem Cells in Culture
A Functional Genomics Approach to Hematopoietic Stem Cell Regulation