Xiao W. (ed.) Yeast Protocols

4th Edition. — Springer, 2021. — 259 p. — (Methods in Molecular Biology 2196). — ISBN: 978-1-0716-0867-8.8-5.This fourth edition provides all new methods on yeast that have not been included in the past three editions. Divided in four parts, chapters guide readers through general applications for genetic manipulation in yeast cells, genome-wide studies, systematically rearrange the yeast chromosomes, and pathway methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Yeast Protocol, Fourth Edition aims to compliment previous editions while offering new tools for the yeast research community.Use of Yeast Plasmids: Transformation and Inheritance Assays
Construction of Tight Conditional Mutants Using the Improved Auxin-Inducible Degron (iAID) Method in the Budding Yeast Saccharomyces cerevisiae
CRISPR Nickase-Mediated Base Editing in Yeast
Genomic Promoter Shuffling by Using Recyclable Cassettes
Study Essential Gene Functions by Plasmid Shuffling
Scarless Genomic Protein Labeling in Saccharomyces cerevisiae
High-Copy Yeast Library Construction and High-Copy Rescue Genetic Screen in Saccharomyces cerevisiae
Genome-Wide Imaging-Based Phenomic Screening Using Yeast (Saccharomyces cerevisiae) Strain Collections
Applications of Oxford Nanopore Sequencing in Schizosaccharomyces pombe
Measuring Genome-Wide Nascent Nucleosome Assembly Using ReIN-Map
Targeted Integration of Complex Genetic Elements at Multi-Copy Loci by Golden Gate Assembly
Chromosomal Rearrangements of Synthetic Yeast by SCRaMbLE
Use YeastFab to Construct Genetic Parts and Multicomponent Pathways for Metabolic Engineering
A Versatile Protocol to Generate Translocations in Yeast Genomes Using CRISPR/Cas9
Yeast Nucleoplasmic Extracts and an Application to Visualize Chromatin Assembly on Single Molecules of DNA
Assays for Autophagy III: Observing Dynamic Protein Trafficking
Isolation of Aged Yeast Cells Using Biotin-Streptavidin Affinity Purification
High-Throughput Rapid Yeast Chronological Lifespan Assay
Long-Term Imaging and Dynamic Analysis of Cytoophidia in Yeast
Monitoring 5′-End Resection at Site-Specific Double-Strand Breaks by Southern Blot Analysis