Moll Jürgen, Carott, Sebastian (eds.) Target Identification and Validation in Drug Discovery: Methods and Protocols

2nd edition. — Humana Press, 2019. — 316 p. — (Methods in Molecular Biology 1953). — ISBN: 978-1-4939-9144-0.This second edition book explores breakthrough technologies in the field of drug target identification and validation. The volume emphasizes particularly revolutionary technologies, such as CRISPR-related screening, “big data,” and in silico approaches, as well as in vivo applications of CRISPR and best uses of animal models in drug development. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Fully updated and authoritative, Target Identification and Validation in Drug Discovery: Methods and Protocols, Second Edition is an ideal guide for molecular and cellular biologists, pharmacologists, pathologists, bioinformaticians, clinical researchers, or investigators, as well as experts in other fields that need a quick overview of these state-of-the-art technologies.Identification of Novel Targets Using High Throughput Screening Assays
Using Functional Genetics in Haploid Cells for Drug Target Identification
Unbiased Forward Genetic Screening with Chemical Mutagenesis to Uncover Drug–Target Interactions
Integration of RNAi and Small Molecule Screens to Identify Targets for Drug Development
CellProfiler and KNIME: Open-Source Tools for High-Content Screening
Drug Target Profiling and Validation
In Silico Target Druggability Assessment: From Structural to Systemic Approaches
In Silico Drug–Target Profiling
Locus-Specific Knock-In of a Degradable Tag for Target Validation Studies
Expression of Human VH Single Domains as Fc Fusions in Mammalian Cells
Three Dimensional Cell Culture Techniques Mimicking the Tumor Microenvironment In Vitro
The Neurosphere Assay (NSA) Applied to Neural Stem Cells (NSCs) and Cancer Stem Cells (CSCs)
3D-3 Tumor Models in Drug Discovery for Analysis of Immune Cell Infiltration
Establishment and Analysis of a 3D Co-Culture Spheroid Model of Pancreatic Adenocarcinoma for Application in Drug Discovery
Animal Models for the Study of Gene Function In Vivo
In Vivo Pharmacology Models for Cancer Target Research
Use of CRISPR/Cas9 for the Modification of the Mouse Genome
Assessment of Gene Function of Mouse Innate Lymphoid Cells for In Vivo Analysis Using Retroviral Transduction
Creation of PDX-Bearing Humanized Mice to Study Immunooncology
Immunophenotyping of Tissue Samples Using Multicolor Flow Cytometry
Translational Methods to Validate Biomarkers
Combined MicroRNA In Situ Hybridization and Immunohistochemical Detection of Protein Markers
Tangential Flow Filtration with or Without Subsequent Bind-Elute Size Exclusion Chromatography for Purification of Extracellular Vesicles
Chromogenic Tissue-Based Methods for Detection of Gene Amplifications (or Rearrangements) Combined with Protein Overexpression in Clinical Samples