Islam M. Tofazzal, Bhowmik Pankaj K., Molla Kutubuddin A. (eds.) CRISPR-Cas Methods

Humana Press, 2020. — 288 p. — (Springer Protocols Handbooks). — ISBN: 978-1-0716-0615-5.This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template. Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism.Wide Horizons of CRISPR-Cas-Derived Technologies for Basic Biology, Agriculture, and Medicine
Efficient Homologous Recombination-Mediated in Planta Gene Targeting by Egg-Cell-Specific Expression of Staphylococcus aureus Cas9 from Arabidopsis
Rice Gene Knockout or Downregulation through CRISPR-Cas9
CRISPR-Cas-Mediated Single Base Editing at More than One Locus in Rice Genome
CRISPR-Cas9-Mediated Genome Editing of the Model Grass Species Brachypodium distachyon
CRISPR-Cas9-Mediated Gene Editing of the Plant Pathogenic Oomycete Phytophthora palmivora
An Effective CRISPR-Cas9 Technology for Efficiently Isolating Transgene-Free Mutants in Arabidopsis, Brassica napus, Strawberry, and Soybean
An Optimized RNA-Guided Cas9 System for Efficient Simplex and Multiplex Genome Editing in Barley (Hordeum vulgare L.)
Genome Editing of Mammalian Cells Using CRISPR-Cas: From In Silico Designing to In-Culture Validation
Cloning-Free (DNA-Free) CRISPR-Cas9-Mediated Gene Editing in Human Liver Cell Line and Its Detection
A Procedure to Design Guide RNA, Assemble Fragments, and Detect Mutation for Genome Editing in Flax
Delivery of Cas9/sgRNA RNP into Wheat Microspores Using Synthetic CPP for Genome Editing and Gene Expression Modulation
CRISPR-Cas9-Mediated Gene Editing in Wheat: A Step-by-Step Protocol
CRISPR-Cas9-Based Genome Editing of Banana
Homology-Directed Transgene-Free Gene Editing in Chlamydomonas reinhardtii