Wolkers Willem F., Oldenhof Harriëtte (eds.) Cryopreservation and Freeze-Drying Protocols

4th edition. — Springer, 2021. — 730 p. — (Methods in Molecular Biology 2180). — ISBN: 978-1-0716-0782-4.This fourth edition explores fully up-to-date standardly used cryopreservation, vitrification, and freeze-drying protocols for specimens that are used for research purposes, conservation of genetic reserves, and applications in agriculture and medicine. Beginning with a section on the fundamentals as well as the use of mathematical modeling to solve cryobiological problems, the book continues with sections on technological aspects of freezing and drying, analytical methods to study protectant loading of cells and tissues, cell behavior during freezing and drying, and thermodynamic properties of preservation solutions, as well as cryopreservation, vitrification, and freeze-drying protocols for a wide variety of samples and different applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and authoritative, Cryopreservation and Freeze-Drying Protocols, Fourth Edition serves as an ideal guide for researchers and technical assistants in academia and industry with a background in life sciences, medicine, or engineering who want to investigate freezing and drying of biological systems or set up methods to safely store biological specimens while maintaining their function upon reconstitution.Fundamental Aspects of Cryopreservation and Freeze-Drying
Principles Underlying Cryopreservation and Freeze-Drying of Cells and Tissues
Principles of Ice-Free Cryopreservation by Vitrification
The Principles of Freeze-Drying and Application of Analytical Technologies
Mathematical Modeling and Optimization of Cryopreservation in Single Cells
Mathematical Modeling of Protectant Transport in Tissues
Technologies and Methods to Study Freezing and Drying
Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation
Microwave- and Laser-Assisted Drying for the Anhydrous Preservation of Biologics
High-Speed Video Cryomicroscopy for Measurement of Intracellular Ice Formation Kinetics
Use of Ice Recrystallization Inhibition Assays to Screen for Compounds That Inhibit Ice Recrystallization
DSC Analysis of Thermophysical Properties for Biomaterials and Formulations
Osmometric Measurements of Cryoprotective Agent Permeation into Tissues
Use of X-Ray Computed Tomography for Monitoring Tissue Permeation Processes
Use of In Situ Fourier Transform Infrared Spectroscopy in Cryobiological Research
Raman Cryomicroscopic Imaging and Sample Holder for Spectroscopic Subzero Temperature Measurements
Cryopreservation and Freeze-Drying Protocols
Cryopreservation of Semen from Domestic Livestock: Bovine, Equine, and Porcine Sperm
Cryopreservation of Avian Semen
Cryopreservation of Mouse Sperm for Genome Banking
Cryopreservation of Marine Invertebrates: From Sperm to Complex Larval Stages
Aseptic Cryoprotectant-Free Vitrification of Human Spermatozoa by Direct Dropping into a Cooling Agent
Cryopreservation of Mammalian Oocytes: Slow Cooling and Vitrification as Successful Methods for Cryogenic Storage
Vitrification of Porcine Oocytes and Zygotes in Microdrops on a Solid Metal Surface or Liquid Nitrogen
Cryopreservation and Transplantation of Laboratory Rodent Ovarian Tissue for Genome Banking and Biomedical Research
Cryopreservation and Thawing of Human Ovarian Cortex Tissue Slices
Vitrification: A Simple and Successful Method for Cryostorage of Human Blastocysts
Vitrification of Equine In Vivo-Derived Embryos After Blastocoel Aspiration
Frozen Blood Reserves
Isolation, Cryopreservation, and Characterization of iPSC-Derived Megakaryocytes
Chemically Defined, Clinical-Grade Cryopreservation of Human Adipose Stem Cells
Chemically Defined and Xeno-Free Cryopreservation of Human-Induced Pluripotent Stem Cells
Protocol for Cryopreservation of Endothelial Monolayers
Vitrification of Heart Valve Tissues
Cryopreservation of Algae
Cryopreservation of Fern Spores and Pollen
Cryopreservation of Plant Cell Lines Using Alginate Encapsulation
Cryopreservation of Plant Shoot Tips of Potato, Mint, Garlic, and Shallot Using Plant Vitrification Solution 3
Cryopreservation of Seeds and Seed Embryos in Orthodox-, Intermediate-, and Recalcitrant-Seeded Species
Freeze-Drying of Proteins
Freeze-Drying of Lactic Acid Bacteria: A Stepwise Approach for Developing a Freeze-Drying Protocol Based on Physical Properties
Preservation of Mammalian Sperm by Freeze-Drying
Freeze-Drying of Decellularized Heart Valves for Off-the-Shelf Availability