Vemuri Mohan C, Chase Lucas G., Rao Mahendra S. (eds.) Mesenchymal Stem Cell Assays and Applications

Humana Press, 2011. — 552 p. — (Methods in Molecular Biology 698). — ISBN: 978-1-60761-998-7.Mesenchymal Stem Cells have seen an unprecedented level of interest in the last decade, primarily due to their relative ease of isolation, the large numbers of cells present in the adult, and the ability to propagate these cells in culture. In Mesenchymal Stem Cell Assays and Applications, expert researchers from across the globe explore the latest techniques to propagate, characterize, and engineer this special cell type. Chapters outline a set of protocols and assays used by leading investigators in the field, providing standards that can be applied by all researchers to the population of cells used in their experiments. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Ground-breaking and current, Mesenchymal Stem Cell Assays and Applications is a necessary handbook for all researchers working with this ambiguous population of cells.Mesenchymal Stem Cell Assays and Applications
Isolation and Expansion of Mesenchymal Stem Cells/Multipotential Stromal Cells from Human Bone Marrow
Standardized Isolation of Human Mesenchymal Stromal Cells with Red Blood Cell Lysis
Isolation and Growth of Adipose Tissue-Derived Stem Cells
Isolation, Culture, and Characterization of Human Umbilical Cord Stroma-derived Mesenchymal Stem Cells
The Isolation and Culture of Human Cord Blood-Derived Mesenchymal Stem Cells Under Low Oxygen Conditions
Amniotic and Placental Mesenchymal Stem Cell Isolation and Culture
Manufacture of Clinical Grade Human Placenta-Derived Multipotent Mesenchymal Stromal Cells
A Method to Isolate and Culture Expand Human Dental Pulp Stem Cells
Isolation and Culture of Human Multipotent Stromal Cells from the Pancreas
Derivation and Characterization of Human ESC-Derived Mesenchymal Stem Cells
Isolation and Culture of Rodent Bone Marrow-Derived Multipotent Mesenchymal Stromal Cells
Cryopreservation and Revival of Mesenchymal Stromal Cells
Dynamic Expansion Culture for Mesenchymal Stem Cells
Ex Vivo Expansion of Human Mesenchymal Stem Cells on Microcarriers
Osteogenic Differentiation of Human Multipotent Mesenchymal Stromal Cells
Assays of Osteogenic Differentiation by Cultured Human Mesenchymal Stem Cells
Bioreactor Cultivation of Functional Bone Grafts
Adipogenic Differentiation of Human Mesenchymal Stem Cells
Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells: Tips and Tricks
Use of Human Mesenchymal Stem Cells as Alternative Source of Smooth Muscle Cells in Vessel Engineering
Dopaminergic Neuronal Differentiation Protocol for Human Mesenchymal Stem Cells
Hepatic Differentiation of Mesenchymal Stem Cells: In Vitro Strategies
Hepatic Transplantation of Mesenchymal Stem Cells in Rodent Animal Models
Phenotypic Analysis and Differentiation of Murine Mesenchymal Stem Cells
Immunohistochemical Analysis of Human Mesenchymal Stem Cells Differentiating into Chondrogenic, Osteogenic, and Adipogenic Lineages
Panel Development for Multicolor Flow-Cytometry Testing of Proliferation and Immunophenotype in hMSCs
Simplified PCR Assay for Detecting Early Stages of Multipotent Mesenchymal Stromal Cell Differentiation
Transcriptome Analysis of Common Gene Expression in Human Mesenchymal Stem Cells Derived from Four Different Origins
Comparison of Microarray and Quantitative Real-Time PCR Methods for Measuring MicroRNA Levels in MSC Cultures
Two Dimensional Gel Electrophoresis Analysis of Mesenchymal Stem Cells
Proteomic Analysis of Human Mesenchymal Stem Cells
Metabolic Labeling and Click Chemistry Detection of Glycoprotein Markers of Mesenchymal Stem Cell Differentiation
BacMam-Mediated Gene Delivery into Multipotent Mesenchymal Stromal Cells
Cell Surface Engineering of Mesenchymal Stem Cells