Ji Yinduo (ed.) Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols: Cutting-Edge Technologies and Advancements

3rd edition. — Humana Press, 2020. — 274 p. — (Methods in Molecular Biology 2069). — ISBN: 978-1-4939-9848-7.This third edition volume expands on the previous editions with an update on the latest techniques used for the detection, genotyping, and investigating pathogenesis of Staphylococcus aureus in vitro and in vivo. The methods covered in this book mostly focus on routine clinical diagnosis, surveillance, research, and practice for treatment of patients infected by multi-drug resistant S. aureus. The book also covers the epidemiology of MRSA, molecular typing approaches, clinical treatment of MRSA infections, and animal models of drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Informative and cutting-edge, Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols: Cutting-Edge Technologies and Advancements, Third Edition is a valuable resource for researchers looking to set up new methods to study S. aureus, and will also be very useful for technicians and scientists working on other bacterial pathogens.Clinical, Epidemiologic, and Laboratory Aspects of Methicillin-Resistant Staphylococcus aureus Infections
Rapid Methods for Detection of MRSA in Clinical Specimens
Immunofluorescence Microscopy for the Detection of Surface Antigens in Methicillin Resistant Staphylococcus aureus (MRSA)
Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA
Pulsed-Field Gel Electrophoresis Typing of Staphylococcus aureus Strains
Spa Typing of Staphylococcus aureus Isolates
Multilocus Sequence Typing of Staphylococcus aureus
Genetic Manipulations of Staphylococcal Chromosomal DNA
Genetic Manipulation of MRSA Using CRISPR/Cas9 Technology
Identification of Target Genes Mediated by Two-Component Regulators of Staphylococcus aureus Using RNA-seq Technology
Application of Two-Dimensional Difference Gel Electrophoresis in Identification of Factors Responsible for Virulence of Staphylococcus aureus
Identification of Virulence Determinants During Host-Pathogen Interaction Using Tn-Seq Technology
Metabolomic Profiling of Staphylococcus aureus
Determining Impact of Growth Phases on Capacity of Staphylococcus aureus to Adhere to and Invade Host Cells
Preclinical Models and Methodologies for Monitoring Staphylococcus aureus Infections Using Noninvasive Optical Imaging
Methicillin-Resistant Staphylococcus aureus Infection and Treatment Options
Animal Models for Drug Development for MRSA