Shrestha Bindesh (ed.) Single Cell Metabolism: Methods and Protocols

Humana Press, 2020. — 230 p. — (Methods in Molecular Biology 2064) — ISBN: 978-1-4939-9829-6This volume explores the latest techniques and workflow for the analysis of single cells metabolism. The chapters in this book cover topics such as the development of mass spectrometry-based single cell approaches, Pico-ESI-MS for single-cell metabolomics analysis; laser capture microdissection; ambient single cell metabolite profile (DESI and LAESI); and MALDI-MS methodology, quantum dots for quantitative cytology to study metabolic heterogeneity of single cells. Written in the highly successful Methods in Molecular Biology series format, the chapters consist of introductions to the topic, lists of the necessary materials and reagents, step-by-step guidelines, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Single Cell Metabolism: Methods and Protocols is a valuable resource for any researcher and scientist interested in learning more about this field.Single-Cell Metabolomics by Mass Spectrometry
Identification of Metabolites in Single Cells by Ion Mobility Separation and Mass Spectrometry
Analysis of Lipids in Single Cells and Organelles Using Nanomanipulation-Coupled Mass Spectrometry
Development of Pico-ESI-MS for Single-Cell Metabolomics Analysis
Single-Probe Mass Spectrometry Analysis of Metabolites in Single Cells
Applications of MicroArrays for Mass Spectrometry (MAMS) in Single-Cell Metabolomics
Laser Capture Microdissection–Liquid Vortex Capture Mass Spectrometry Metabolic Profiling of Single Onion Epidermis and Microalgae Cells
Single Cell Analysis by High-Resolution Atmospheric-Pressure MALDI MS Imaging
A MALDI-MS Methodology for Studying Metabolic Heterogeneity of Single Cells in a Population
Sample Preparation and Analysis of Single Cells Using High Performance MALDI FTICR Mass Spectrometry
Toward Single Cell Molecular Imaging by Matrix-Free Nanophotonic Laser Desorption Ionization Mass Spectrometry
Compact Quantum Dots for Quantitative Cytology
Ambient Lipidomic Analysis of Single Mammalian Oocytes and Preimplantation Embryos Using Desorption Electrospray Ionization (DESI) Mass Spectrometry
Spatial Mapping of Cellular Metabolites Using DESI Ion Mobility Mass Spectrometry
Open-Source Software Tools, Databases, and Resources for Single-Cell and Single-Cell-Type Metabolomics
Ten Major Future Challenges in Single-Cell Metabolomics