Proft Thomas, Loh Jacelyn M.S. (Eds.) Group A Streptococcus: Methods and Protocols

Humana Press, 2020. — 417 p. — (Methods in Molecular Biology 2136). — ISBN: 978-1071604663This volume details Group A Streptococcus (GAS) research and provides the reader with an extensive collection of research protocols within this important field. Chapters guide readers through standard genetic protocols such as whole genome sequencing, transcriptome analyses, proteome analysis, application of GAS-derived molecular tools, and methods that are crucial for the evaluation of novel GAS vaccines and GAS virulence factors, including bactericidal assays and animal infection models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Detection of Streptococcus pyogenes Virulence Factors
Closed-Tube Multiplex Real-Time PCR for the Detection of Group A Streptococcal Superantigens
The emm-Cluster Typing System
Protocols for Tn-seq Analyses in the Group A Streptococcus
Genetic Manipulation of Group A Streptococcus—Gene Deletion by Allelic Replacement
Generation of Bioluminescent Group A Streptococcus for Biophotonic Imaging
Whole Genome Sequence Analysis and Population Genomics of Group A Streptococci
Group A Streptococcus Transcriptome Analysis
Protocol for Proteome Analysis of Group A Streptococcus
Investigation of Group A Streptococcal Interactions with Host Glycan Structures Using High-Throughput Techniques: Glycan Microarray Analysis Using Recombinant Protein and Whole Cells
Using Lactococcus lactis as Surrogate Organism to Study Group A Streptococcus Surface Proteins
Expression and Purification of Collagen-Like Proteins of Group A Streptococcus
Detection of Fibronectin-Binding Proteins of Streptococcus pyogenes Using Ligand Blot Analysis
Morphology and Ultrastructure of Group A Streptococcus Biofilms
Dynamic Interactions of Group A Streptococcus with Host Macrophages
Identification of Group A Streptococcus-Containing Autophagosome-Like Vacuoles
Flow Cytometry-Based Assays to Quantify Complement Deposition and Neutrophil Uptake of Group A Streptococcus
Live Cell Microscopy and Flow Cytometry to Study Streptolysin S-Mediated Erythrocyte Hemolysis
Isolation of Monoclonal Antibodies to Group A Streptococcus Antigens Using Phage Display
Assays to Analyze Adhesion of Group A Streptococcus to Host Cells
The Use of Galleria mellonella (Wax Moth) as an Infection Model for Group A Streptococcus
A Superficial Skin Scarification Method in Mice to Mimic Streptococcus pyogenes Skin Infection in Humans
A Mouse Nasopharyngeal Colonization Model for Group A Streptococcus
Humanized Plasminogen Mouse Model to Study Group A Streptococcus Invasive Disease
Lancefield Whole Blood Killing Assay to Evaluate Vaccine Efficacy
An Opsonophagocytic Killing Assay for the Evaluation of Group A Streptococcus Vaccine Antisera
Validation of Suitable Carrier Molecules and Target Genes for Antisense Therapy Using Peptide-Coupled Peptide Nucleic Acids (PNAs) in Streptococci
Interfering with the Folding of Group A Streptococcal pili Proteins
Use of Streptolysin O (SLO) to Study the Function of Lipid Rafts
Engineering of Group A Streptococcus Isopeptide Bonds into Immunoglobulin-Like Protein Domains