Tonkin C.J. (Ed.) Toxoplasma gondii: Methods and Protocols

Springer, 2020. — 469 p. – (Methods in Molecular Biology 2071). — ISBN: 978-1-4939-9856-2.This volume covers a diverse collection of protocols currently being used by the Toxoplasma research community, and also looks at innovative methods that are pushing the boundaries of possibilities. Chapters in this book discuss topics such as isolation and genotyping of Toxoplasma gondii strains; assessing rhoptry secretion in T. gondii; plate-based quantification of stimulated Toxoplasma egress; methods to study ocular toxoplasmosis; and metabolic analysis of Toxoplasma gondii tachyzoites. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Toxoplasma gondii: Methods and Protocols is a valuable resource for students or groups starting off in the field, as well as laboratories interested in implementing the latest techniques described in the book.What a Difference 30 Years Makes! A Perspective on Changes in Research Methodologies Used to Study Toxoplasma gondii
ToxoDB: Functional Genomics Resource for Toxoplasma and Related Organisms
Isolation and Genotyping of Toxoplasma gondii Strains
Generation of Toxoplasma gondii and Hammondia hammondi Oocysts and Purification of Their Sporozoites for Downstream Manipulation
Assays for Monitoring Toxoplasma gondii Infectivity in the Laboratory Mouse
PCR Screening of Toxoplasma gondii Single Clones Directly from 96-Well Plates Without DNA Purification
CRISPR/Cas9-Mediated Generation of Tetracycline Repressor-Based Inducible Knockdown in Toxoplasma gondii
Assessing Rhoptry Secretion in T. gondii
High-Throughput Measurement of Microneme Secretion in Toxoplasma gondii
Plate-Based Quantification of Stimulated Toxoplasma Egress
Genetic Indicators for Calcium Signaling Studies in Toxoplasma gondii
Phenotyping Toxoplasma Invasive Skills by Fast Live Cell Imaging
Experimental Approaches for Examining Apicoplast Biology
Measuring Solute Transport in Toxoplasma gondii Parasites
Toxoplasma gondii: Bradyzoite Differentiation In Vitro and In Vivo
Three-Dimensional Reconstruction of Toxoplasma–Neuron Interactions In Situ
Model Systems for Studying Mechanisms of Ocular Toxoplasmosis
Using BioID for the Identification of Interacting and Proximal Proteins in Subcellular Compartments in Toxoplasma gondii
Assays to Evaluate Toxoplasma–Macrophage Interactions
Methods for the Measurement of Early Events in Toxoplasma gondii Immunity in Mouse Cells
Image-Based Quantitation of Host Cell–Toxoplasma gondii Interplay Using HRMAn: A Host Response to Microbe Analysis Pipeline
Metabolomic Analysis of Toxoplasma gondii Tachyzoites
Label-Based Mass Spectrometry Approaches for Robust Quantification of the Phosphoproteome and Total Proteome in Toxoplasma gondii