Colgan S.P. (Ed.) Cell-Cell Interactions: Methods and Protocols

Humana Press Inc., 2006. — 306 p. — (Methods in Molecular Biology 341). — ISBN: 1588295230.A versatile collection of readily reproducible cell-cell interaction assays for uncovering cellular interactions at the molecular level, both in vitro and in vivo. The protocols cover a diverse set of cell-cell interaction models in both normal and pathological states, are readily adaptable to nearly any cell type and organ system, and include primary data and outcome analysis. In addition, the protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.Blood–Brain Barrier and Cell–Cell Interactions: Methods for Establishing In Vitro Models of the Blood–Brain Barrier and Transport Measurements
Interactions of Intestinal Epithelial Cells With Bacteria and Immune Cells: Methods to Characterize Microflora and Functional Consequences
The Role of Junctional Adhesion Molecules in Interactions Between Vascular Cells
Cell–Cell Interactions in the Kidney: Inducible Expression of Mutant G Protein α-Subunits in Madin–Darby Canine Kidney Cells for Studies of Epithelial Cell Junction Structure and Function
Nucleotide Metabolism and Cell–Cell Interactions
Analysis of Mammalian Sperm–Egg Membrane Interactions During In Vitro Fertilization
Collagen Gel Contraction Assay Peter Ngo, Punitha Ramalingam, Jonathan A. Phillips,
Methods to Assess Tissue Permeability
Bacterial–Bacterial Cell Interactions in Biofilms: Detection of Polysaccharide Intercellular Adhesins by Blotting and Confocal Microscopy
A Biochemical Method for Tracking Cholera Toxin Transport From Plasma Membrane to Golgi and Endoplasmic Reticulum
Isolation and Culture of Murine Heart and Lung Endothelial Cells for In Vitro Model Systems
Expression Cloning of Signaling Proteins Regulated
Assays for the Calcium Sensitivity of Desmosomes
Tight Junctions and Cell–Cell Interactions
Simultaneous Immunofluorescence Detection of Cadherin and β-Catenin in Mouse Tissue Sections
In Vitro Neutrophil Transepithelial Migration
The Blot Rolling Assay: A Method for Identifying Adhesion Molecules Mediating Binding Under Shear Conditions
Cell–Cell Interaction in the Transcellular Biosynthesis of Novel ω-3-Derived Lipid Mediators
Isolation and Analysis of Lipid Rafts in Cell–Cell Interactions