Turksen K. (Ed.) Stem Cells and Aging: Methods and Protocols

2nd Edition. — Humana Press, 2019. — 353 p. — (Methods in Molecular Biology 2045). — ISBN: 978-1-4939-9712-1.This fully updated book collects protocols that reflect current investigations into how stem cell populations change during organismal aging. These methodologies are well-established and described in easy to follow fashion so as to be valuable for not only experts but novices in the ever-developing stem cell field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Stem Cells and Aging: Methods and Protocols, Second Edition serves as an ideal guide for all researchers working in this very active area of study.Assessing Muscle Stem Cell Clonal Complexity During Aging
Simultaneous Isolation of Stem and Niche Cells of Skeletal Muscle: Applicability for Aging Studies
Isolation and Culture of Individual Myofibers and Their Adjacent Muscle Stem Cells from Aged and Adult Skeletal Muscle
Methods and Strategies for Procurement, Isolation, Characterization, and Assessment of Senescence of Human Mesenchymal Stem Cells from Adipose Tissue
Quantifying Senescence-Associated Phenotypes in Primary Multipotent Mesenchymal Stromal Cell Cultures
Adipogenic and Osteogenic Differentiation of In Vitro Aged Human Mesenchymal Stem Cells
Human Skeletal Muscle-Derived Mesenchymal Stem/Stromal Cell Isolation and Growth Kinetics Analysis
Complete Assessment of Multilineage Differentiation Potential of Human Skeletal Muscle-Derived Mesenchymal Stem/Stromal Cells
Human Synovium-Derived Mesenchymal Stem Cells: Ex Vivo Analysis
3D-Embedded Cell Cultures to Study Tendon Biology
Targeted, Amplicon-Based, Next-Generation Sequencing to Detect Age-Related Clonal Hematopoiesis
Column-Free Method for Isolation and Culture of C-Kit Positive Stem Cells from Atrial Explants
Histological Assessment of Cre-loxP Genetic Recombination in the Aging Subventricular Zone of Nestin-CreERT2/Rosa26YFP Mice
Infrared Spectroscopy and Imaging in Stem Cells and Aging Research
Use of U-STELA for Accurate Measurement of Extremely Short Telomeres
Surface Antigen-Based Identification of In Vitro Expanded Skeletal Muscle-Derived Mesenchymal Stromal/Stem Cells Using Flow Cytometry
Analysis of Stem Cells and Their Activity in Human Skeletal Muscles by Immunohistochemistry
Methods for Detection of Autophagy in Mammalian Cells
Metabolomic and Proteomic Analyses of Mouse Primordial Germ Cells
Reprogramming of Aged Cells into Pluripotent Stem Cells by Nuclear Transfer
Generation of Transplantable Retinal Pigmented Epithelial (RPE) Cells for Treatment of Age-Related Macular Degeneration (AMD)
Histopathological and Behavioral Assessments of Aging Effects on Stem Cell Transplants in an Experimental Traumatic Brain Injury
3D Age-Specific Mortality Trajectory: A Survival Analysis Protocol
Isolation, Expansion, and Characterization of Wharton’s Jelly-Derived Mesenchymal Stromal Cell: Method to Identify Functional Passages for Experiments
CRISPR Base Editing in Induced Pluripotent Stem Cells