Richard Guy-Franck (ed.) Trinucleotide Repeats: Methods and Protocols

Humana Press, 2019. — 292 p. — (Methods in Molecular Biology 2056) — ISBN: 978-1-4939-9783-1This detailed book contains techniques to explore the unusual properties of the peculiar microsatellites known as trinucleotide repeats. Beginning with technical challenges raised by secondary structure-forming repeats and their propensity to contract and expand over time, the book continues with genetic screens in Saccharomyces cerevisiae, experimental systems to study trinucleotide repeat instability in human cells, dedicated experimental systems in appropriate cell types, as well as gene therapy approaches using the CRISPR-Cas family of endonucleases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Trinucleotide Repeats: Methods and Protocols serves as a valuable aid to experts and newcomers alike who seek to investigate this fascinating and ever-expanding field of study.Experimenting with Trinucleotide Repeats: Facts and Technical Issues
Fast Assays to Detect Interruptions in CTG.CAG Repeat Expansions
Tracking Expansions of Stable and Threshold Length Trinucleotide Repeat Tracts In Vivo and In Vitro Using Saccharomyces cerevisiae
Quantifying Replication Fork Progression at CTG Repeats by 2D Gel Electrophoresis
Genetic Assays to Study Repeat Fragility in Saccharomyces cerevisiae
Genetic Screens to Study GAA/TTC and Inverted Repeat Instability in Saccharomyces cerevisiae
Monitoring Double-Strand Break Repair of Trinucleotide Repeats Using a Yeast Fluorescent Reporter Assay
Analysis of Trinucleotide Repeat Stability by Integration at a Chromosomal Ectopic Site
Experimental System to Study Instability of (CGG)n Repeats in Cultured Mammalian Cells
Assessing Triplet Repeat Expansions in Human SVG-A Cell Culture
Isolation and Analysis of the CGG-Repeat Size in Male and Female Gametes from a Fragile X Mouse Model
In Vitro Synthesis and RNA Structure Probing of CUG Triplet Repeat RNA
FISH Protocol for Myotonic Dystrophy Type 1 Cells
Real Time Videomicroscopy and Semiautomated Analysis of Brain Cell Culture Models of Trinucleotide Repeat Expansion Diseases
Primary Cultures of Pure Embryonic Dorsal Root Ganglia Sensory Neurons as a New Cellular Model for Friedreich’s Ataxia
GFP Reporters to Monitor Instability and Expression of Expanded CAG/CTG Repeats
Gene Therapy for Huntington’s Disease Using Targeted Endonucleases