Stocker Claire J. (ed.) Type 2 Diabetes: Methods and Protocols

2nd edition. — Humana Press, 2020. — 300 p. — (Methods in Molecular Biology 2076) — ISBN: 978-1-4939-9880-7.This second edition volume expands on the previous editions with updates on the commonly used techniques and analysis used by clinicians and scientists to study diabetes. The chapters in this book cover topics such as nutritional models of type 2 diabetes mellitus; the isolation and purification of rodent pancreatic islets of Langerhans; nitric oxide and redox state measurements in pancreatic beta cells; and primary adipocytes as a model for insulin sensitivity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and practical, Type 2 Diabetes: Methods and Protocols, Second Edition is a valuable resource for all researchers who are interested in learning more about this important and evolving field.Sixty Years of Drug Discovery for Type 2 Diabetes: Where Are We Now?
Practical Considerations for In Vivo Mouse Studies
Nutritional Models of Type 2 Diabetes Mellitus
Cheminformatics in the Identification of Drug Classes for the Treatment of Type 2 Diabetes
Whole-Exome Sequencing (WES) for Illumina Short Read Sequencers Using Solution-Based Capture
Gene Expression Mining in Type 2 Diabetes Research
Pathways Enrichment Analysis of Gene Expression Data in Type 2 Diabetes
Diagnostic Genetic Testing for Monogenic Diabetes and Congenital Hyperinsulinemia
Isolation and Purification of Rodent Pancreatic Islets of Langerhans
Characterization of Islet Leukocyte Populations in Human and Murine Islets by Flow Cytometry
Analysis of Histone Modifications in Rodent Pancreatic Islets by Native Chromatin Immunoprecipitation
Quantification of Pancreatic Islets: Using Image Analysis Tools
Measurement of Calcium Signaling in Beta-Cell Lines Using Epifluorescence and Confocal Microscopy
Nitric Oxide and Redox State Measurements in Pancreatic Beta Cells
Primary Adipocytes as a Model for Insulin Sensitivity
Assessing Islet Transplantation Outcome in Mice
Measurement of Insulin Secretion Using Pancreas Perfusion in the Rodent