Li S., Chang L., Teissie J. (Eds.) Electroporation Protocols: Microorganism, Mammalian System, and Nanodevice

3rd Edition. — 2019. — 191 p. — (Methods in Molecular Biology 2050). — ISBN: 1493997394.This third edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted microorganism, mammalian single cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on the progress made in siRNA delivery and DNA vaccines for large animals as well as discovery of electroporation applications for the fragile tissues and for internal organs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Electroporation Protocols: Microorganism, Mammalian System, and Nanodevice, Third Edition aims to be an invaluable resource for investigators both in and outside of this field.Design and Fabrication of Micro-/Nano-Electroporation
Cell Size-Specific Transfection by Micropillar Array Electroporation
Soft Electroporation Through 3D Hollow Nanoelectrodes
Micromachining of Polymeric Microfluidic Micro/Nanoelectroporation Device
3D Nanochannel Array for High-Throughput Cell Manipulation and Electroporation
Low-Voltage Flow-Through Electroporation Membrane and Method
Micro-/Nano-Electroporation for in Vitro Delivery
Nanofountain Probe Electroporation for Monoclonal Cell Line Generation
3D Nanochannel Electroporation for Macromolecular Nucleotide Delivery
Nanoelectroporation and Collection of Genetically Modified Exosomes in Primary Cultures of Dendritic Cells
Electroporation of CRISPR-Cas9 into Malignant B Cells for Loss-of-Function Studies of Target Gene Via Knockout
Microfluidic Device for Localized Electroporation
Advanced in Vivo Electroporation
Transdermal Delivery of Nucleic Acid Mediated by Punching and Electroporation
Targeted In Vivo Electroporation Using Nanoengineered Microelectrodes
Zygote Electroporation for CRISPR/Cas9 Delivery to Generate Genetically Modified Mice
Genome Editing in Staphylococcus aureus by Conditional Recombineering and CRISPR/Cas9-Mediated Counterselection
Isolation and Nanoscale Electroporation of Primary Neuronal Cultures In Situ
Microorganism Electroporation
Transformation of the Model Microalga Chlamydomonas reinhardtii Without Cell-Wall Removal
Electroporation Transformation Protocol for Phaeodactylum tricornutum
Efficient Transformation of the Diatoms Phaeodactylum tricornutum by Multipulse Electroporation
Rapid and Effective Electroporation Protocol for Nannochloropsis oceanica
Electroporation Procedures for Genetic Modification of Green Algae (Chlorella spp.)
Electrotransformation of Saccharomyces cerevisiae