Brown Amanda Claire (ed.) Chlamydia trachomatis: Methods and Protocols

Humana Press, 2019. — 294 p. — (Methods in Molecular Biology 2042). — ISBN: 978-1-4939-9693-3.This book explores cutting-edge methods to work with the notoriously difficult, but highly prevalent, obligate intracellular pathogen, Chlamydia trachomatis. These include techniques to identify Chlamydia trachomatis in patient samples, ranging from simple point-of-care tests to whole genome sequencing; methods for propagation of strains in both cell culture and animal models; techniques to manipulate Chlamydia trachomatis in molecular genetic methodologies; a high-throughput screening method for testing new potential drugs against intracellular bacteria; a screen for antibiotic resistance; methods for labeling and enumeration; and descriptions of genotyping technologies, as well as dual RNA-Seq transcriptional profiling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative, practical, and relevant, Chlamydia trachomatis: Methods and Protocols serves as an ideal reference for scientists searching for a better understanding of the pathogen, allowing for the development of improved treatment regimens and the discovery of new drugs.Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Multiplex Strand Invasion Based Amplification (mSIBA)
Point-of-Care Chlamydia trachomatis Detection Using Loop-Mediated Isothermal Amplification and Hydroxynaphthol Blue
Application of Loop-Mediated Isothermal Amplification Assay for the Detection of Chlamydia trachomatis and Neisseria gonorrhoeae
Simultaneous Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Real-Time Multiplex qPCR Assay
Antimicrobial Resistance Screening in Chlamydia trachomatis by Optimized McCoy Cell Culture System and Direct qPCR-Based Monitoring of Chlamydial Growth
Whole-Genome Sequencing of Chlamydia trachomatis Directly from Human Samples
Multilocus Sequence Typing (MLST) of Chlamydiales
Identification and Discrimination of Chlamydia trachomatis Ocular and Urogenital Strains and Major Phylogenetic Lineages by CtGEM Typing, A Double-Locus Genotyping Method
Dual RNA-Seq of Chlamydia and Host Cells
Isolation and Propagation of Single Inclusion-Derived Chlamydia Using Laser Microdissection
Genetic Manipulation of Chlamydia trachomatis: Chromosomal Deletions
Mutagenesis of Chlamydia trachomatis Using TargeTron
Forward and Reverse Genetic Analysis of Chlamydia
A Coinfection Model to Evaluate Chlamydia Inc Protein Interactions
Rodent Infections for Chlamydia spp.
Enumeration of Viable Chlamydia from Infected Animals Using Immunofluorescent Microscopy
Proximity Labeling of the Chlamydia trachomatis Inclusion Membrane
High-Throughput Screening for Novel Inhibitors of Intracellular Pathogens, Including Chlamydia trachomatis