Li Ka Wan (ed.) Neuroproteomics

2nd edition. — Humana Press, 2019. — 492 p. — (Neuromethods 146) — ISBN: 978-1-4939-9662-9This second edition volume expands on the previous edition with updates on the latest techniques used in the field of proteomics. Tissue and cellular proteins are now being analyzed at near proteome-wide levels and mass spectrometers are achieving higher sensitivity and speed, resolution, and accuracy. The chapters in this book cover various new methods used in proteomic workflows such as laser microdissection for spatial proteomics of post-mortem brain tissue, sample preparation in single vessel with FASP and SP3 protocols, the crosslinking mass spectrometry, TMT-MS3 based proteomic with synchronous precursor selection, mass spectrometric data dependent acquisition (DDA) and data independent acquisition (DIA, SWATH) methods, 2D-DIGE, and MALDI Imaging Mass Spectrometry. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Cutting-edge and comprehensive, Neuroproteomics, Second Edition is a valuable guide that help researchers obtain the finest results from current proteomics platforms.Neuroproteomics: The Methods
Dissection of Rodent Brain Regions: Guided Free-Hand Slicing and Dissection of Frozen Tissue
Isolation of Synapse Sub-Domains by Subcellular Fractionation Using Sucrose Density Gradient Centrifugation: Purification of the Synaptosome, Synaptic Plasma Membrane, Postsynaptic Density, Synaptic Membrane Raft, and Postsynaptic Density Lattice
Laser Microdissection for Spatial Proteomics of Postmortem Brain Tissue
High-pH Reversed-Phase Pre-Fractionation for In-Depth Shotgun Proteomics
Sample Preparation for Proteomics Analysis: Filter-Aided Sample Preparation (FASP) and Single-Pot Solid-Phase Sample Preparation (SP3)
Enrichment of Low-Molecular-Weight Phosphorylated Biomolecules Using Phos-Tag Tip
Integrated Immunoprecipitation: Blue Native Gel Electrophoresis—Mass Spectrometry for the Identification of Protein Subcomplexes
Analysis of Synaptic Protein–Protein Interaction by Cross-linking Mass Spectrometry
TMT-MS3-Enabled Proteomic Quantification of Human IPSC-Derived Neurons
Data-Independent Acquisition (SWATH) Mass Spectrometry Analysis of Protein Content in Primary Neuronal Cultures
Quantitative Analysis of Mass Spectrometry-Based Proteomics Data
2D-DIGE as a Tool in Neuroproteomics
Neuropeptidomics of the Mammalian Brain
MALDI Imaging Mass Spectrometry: Neurochemical Imaging of Proteins and Peptides
A Combined Cellomics and Proteomics Approach to Uncover Neuronal Pathways to Psychiatric Disorder