Burleson Florence G., Chambers Thomas M., Wiedbrauk Danny L. (eds.) Virology: A Laboratory Manual

Academic Press, 1992. — 244 p. — ISBN: 978-0121447304Virology: A Laboratory Manual is designed for a one-semester virology laboratory course, although more than one semester of exercises are included. Choices of experiments allow for flexibility within a sequentially organized framework. The text features detailed experimental protocols with comprehensive sections on materials and preparations for all exercises, plus introductory material, discussion questions, and further reading. the use of few viruses and cell lines provides continuity and simplifies preparation of the laboratory exercises. An Instructor's Manual is available to give alternative and assistance in laboratory set-up.Introduction to the Virology Laboratory
Introduction to Cell Culture
Preparation of Cell Culture Medium
Cell Growth and Cell Passage
Counting Cells with a Hemacytometer
Primary Cell Cultures
Cryopreservation of Cells
Virus Replication in Cell Culture
Virus Replication in Embryonated Eggs
Introduction to Quanta] Virus Assays
TCID-50
LD-50
EID-50
Introduction to Quantitative Assays
Plaque Assays
Hemagglutination Assay
Hemadsorption
Introduction to Virus Characterization
Viral Replication: One-Step Growth Curve
Determination of Virus Size
Assessing Thermostability
Sensitivity to Lipid Solvents
Sensitivity to Low pH
EMC Virus Neutralization
Hemagglutination-Inhibition Assay
Immunofluorescent Staining of EMC Virus-Infected Cells
Enzyme-Linked Immunosorbent Assay (ELISA)