ASTM D 6499 - 16 Standard Test Method for The Immunological Measurement of Antigenic Protein in Natural Rubber and its Products

Current edition approved July 1, 2016. Published August 2016. Originally approved in 2000. Last previous edition approved in 2012 as D6499 – 12. — 9 p.
This test method covers an immunological method to determine the amount of antigenic protein in natural rubber and its products using rabbit antisera specific for natural rubber latex (NRL) proteins. This immunoassay procedure quantitatively measures the level of antigenic latex proteins in solution using an inhibition format. The samples may include glove or other rubber product extracts which have been collected in order to measure the latex protein levels. Although this method detects antigenic proteins, it should not be considered as a measure of allergenic proteins. Correlation of protein/antigen levels with the level of allergenic proteins has not been fully established.