Current edition approved July 1, 2015. Published September 2015. Originally approved in 1995. Last previous edition approved in 2010 as D5712 – 10. — 9 p.
This test method covers an analytical test for determining the amount of total aqueous extractable protein associated with NR, latex, and elastomeric products. Water soluble proteins are extracted in a buffer solution and then precipitated to concentrate them and also to separate them from water soluble substances that may interfere with the determination. The extracted protein is redissolved and quantified colorimetrically by the modified Lowry method using a protein standard.