Sagi I., Afratis N.A. (Eds.) Collagen: Methods and Protocols

Springer, 2019. — 255 p. — (Methods in Molecular Biology 1944). — ISBN: 978-1-4939-9094-8.This detailed volume compiles state-of-the-art protocols that will serve as recipes for scientists researching collagen, an abundant protein with great importance to health and disease, as well as in applications like food, cosmetics, pharmaceuticals, cosmetic surgery, artificial skin, and glue. Beginning with a section on in vitro models for the characterization of collagen formation, the book continues by highlighting large-scale analysis of collagen with mass spectrometry in order to elucidate the proteomics, degradomics, interactomes, and cross-linking of collagen, high resolution imaging approaches for collagen by the use of scanning electron microscopy and multiphoton imaging, as well as the role of collagen during physiological and pathological conditions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Collagen: Methods and Protocols is an ideal guide to high quality and repeatable protocols in this vital field of study.Cloning and Mutagenesis Strategies for Large Collagens
Multi-well Fibrillation Assay as a Tool for Analyzing Crosslinking and Stabilization of Collagen
Production and Characterization of Chemically Cross-Linked Collagen Scaffolds
A Biomimetic Model for Mineralization of Type-I Collagen Fibrils
Investigation of Triple-Helix Collagen Hydroxylation by Solid-State NMR Spectroscopy
Measurement of Collagen Cross-Links from Tissue Samples by Mass Spectrometry
Mass Spectrometry-Based Proteomics to Define Intracellular Collagen Interactomes
Exploring Extracellular Matrix Degradomes by TMT-TAILS N-Terminomics
Sample Preparation of Extracellular Matrix of Murine Colons for Scanning Electron Microscopy
In Situ Detection of Degraded and Denatured Collagen via Triple Helical Hybridization: New Tool in Histopathology
Combination of Traction Assays and Multiphoton Imaging to Quantify Skin Biomechanics
Analysis of Collagen-Binding Integrin Interactions with Supramolecular Aggregates of the Extracellular Matrix
Assessing Collagen Deposition During Aging in Mammalian Tissue and in Caenorhabditis elegans
Procollagen C-Proteinase Enhancer 1 (PCPE-1) in Liver Fibrosis
Tumorigenic Interplay Between Macrophages and Collagenous Matrix in the Tumor Microenvironment
FACS Analysis of Col1α Protein Levels in Primary Fibroblasts
Methods for the Construction of Collagen-Based Triple-Helical Peptides Designed as Matrix Metalloproteinase Inhibitors