Ludwig Maximilian University of Munich, 2014. — 203 p.One of the most frequent ways to interact with the surrounding environment occurs as a visual way. Hence imaging is a very common way in order to gain information and learn from the environment. Particularly in the field of cellular biology, imaging is applied in order to get an insight into the minute world of cellular complexes. As a result, in recent years many researches have focused on developing new suitable image processing approaches which have facilitates the extraction of meaningful quantitative information from image data sets. In spite of recent progress, but due to the huge data set of acquired images and the demand for increasing precision, digital image processing and statistical analysis are gaining more and more importance in this field.The specific contributions of this thesis can be summarized as follows: Substitution of the time-consuming, subjective and laborious task of manual post-picking in Cryo-EM process by a fully automatic particle post-picking routine based on Machine Learning methods (Part I). Quality enhancement of the 3D reconstruction image due to the high performance of automatically post-picking steps (Part I). Developing a full automatic tool for detecting subcellular objects in multichannel 3D Fluorescence images (Part II). Extension of known colocalization analysis by using spatial statistics in order to investigate the surrounding point distribution and enabling to analyze the colocalization in combination with statistical significance (Part II). All introduced approaches are implemented and provided as toolboxes which are free available for research purposes.
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